Recombinant Human CX3CL1/Fractalkine Protein

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Pricing & Details

Product. No.F116
Alternate Names
Fractalkine, CX3CL1, NTN, ABCD-3, C3Xkine, CXC3, CXC3C, NTT, SCYD1, Neurotactin, FKN
Expression Host
NS0 cells
Species
Human
Prod No.
Size
Price
Avail.
Qty
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F116-25 µg
25 µg
$349.00
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Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Background

Fractalkine (FKN) is a transmembrane mucin-chemokine hybrid molecule expressed on activated endothelium1 that mediates attachment and firm adhesion of T cells, monocytes and NK cells.2 FKN plays an important proinflammatory role in rheumatoid arthritis (RA) pathogenesis as characterized by induction of synovial angiogenesis, chemotaxis, activation of monocytes and T cells as well as the stimulation of proliferation and synthesis of matrix degrading enzymes (matrix metalloproteinases, MMP) in synovial fibroblasts. Fractalkine thus may represent a novel target molecule for therapeutic intervention in RA.3 FKN is also an essential biomarker for predicting the prognosis of patients with colorectal cancer (CRC).4

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Fractalkine was determined by its ability to chemoattract freshly isolated peripheral blood lymphocytes or mouse Baf/3 cells transfected with hCX3CR-1. The expected ED50 for this effect is typically 50 - 150 ng/ml, or 2.5 - 10 ng/ml, respectively.
Fusion Protein Tag
Histidine Tag
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
qhhgvtkcni tcskmtskip vallihyqqn qascgkraii letrqhrlfc adpkeqwvkd amqhldrqaa altrnggtfe kqigevkprt tpaaggmdes vvlepeatge sssleptpss qeaqralgts pelptgvtgs sgtrlpptpk aqdggpvgte lfrvppvsta atwqnsaphq pgpslwaeak tseapstqdp stqastassp apeenapseg qrvwgqgqsp rpensleree mgpvpahtda fqdwgpgsma hvsvvpvsse gtpsrepvas gswtpkaeep ihatmdpqrl gvlitpvpda qaatrkqnhh hhhh
N-terminal Sequence Analysis
Gln25 predicted
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human Fractalkine is Mr 35 kDa. However, due to glycosylation, the actual molecular weight as observed by migration on SDS-PAGE is Mr 90 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Patel, DD. et al. (1998) J Exp Med. 188: 1413
2. Hwang, ST. et al. (1999) Eur J Immunol. 29: 2551
3. Muller, GA. et al. (2008) Z Rheumatol. 67: 424
4. Mori, M. et al. (2005) Int J Oncol. 26: 41
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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