Recombinant Human CX3CL1/Fractalkine Protein

Fractalkine (FKN) is a transmembrane mucin-chemokine hybrid molecule expressed on activated endothelium¹ that mediates attachment and firm adhesion of T cells, monocytes and NK cells.² FKN plays an important proinflammatory role in rheumatoid arthritis (RA) pathogenesis as characterized by induction of synovial angiogenesis, chemotaxis, activation of monocytes and T cells as well as the stimulation of proliferation and synthesis of matrix degrading enzymes (matrix metalloproteinases, MMP) in synovial fibroblasts. Fractalkine thus may represent a novel target molecule for therapeutic intervention in RA.³ FKN is also an essential biomarker for predicting the prognosis of patients with colorectal cancer (CRC).⁴

The biological activity of Human Fractalkine was determined by its ability to chemoattract freshly isolated peripheral blood lymphocytes or mouse Baf/3 cells transfected with hCX3CR-1. The expected ED₅₀ for this effect is typically 50 - 150 ng/ml, or 2.5 - 10 ng/ml, respectively.

Histidine Tag

qhhgvtkcni tcskmtskip vallihyqqn qascgkraii letrqhrlfc adpkeqwvkd amqhldrqaa altrnggtfe kqigevkprt tpaaggmdes vvlepeatge sssleptpss qeaqralgts pelptgvtgs sgtrlpptpk aqdggpvgte lfrvppvsta atwqnsaphq pgpslwaeak tseapstqdp stqastassp apeenapseg qrvwgqgqsp rpensleree mgpvpahtda fqdwgpgsma hvsvvpvsse gtpsrepvas gswtpkaeep ihatmdpqrl gvlitpvpda qaatrkqnhh hhhh

Gln25 predicted

Lyophilized

The predicted molecular weight of Recombinant Human Fractalkine is Mr 35 kDa. However, due to glycosylation, the actual molecular weight as observed by migration on SDS-PAGE is Mr 90 kDa.

35

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Patel, DD. et al. (1998) J Exp Med. 188: 1413
2. Hwang, ST. et al. (1999) Eur J Immunol. 29: 2551
3. Muller, GA. et al. (2008) Z Rheumatol. 67: 424
4. Mori, M. et al. (2005) Int J Oncol. 26: 41