Recombinant Human Glial Cell Line-Derived Neurotrophic Factor (GDNF)

Glial cell line-derived neurotrophic factor (GDNF), also known as ATF-1, is a glycosylated, disulfide-bonded homodimer and member of the TGF-β superfamily (1). It is structurally related to artemin, neurturin and persephin. GDNF potently promotes the survival of many types of neurons, most prominently, dopaminergic and motorneurons. It also regulates kidney development, spermatogenesis and it affects alcohol consumption (2). GDNF is expressed in Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. It signals through a multicomponent receptor system composed of a RET oncogene and one of the four GFR-α receptors (3). GDNF protects and repairs dopamine-containing neurons, which degenerate in Parkinson's disease, and motor neurons, which die in amyotrophic lateral sclerosis. The use of GDNF in the treatment of Parkinson's disease has shown promise in the clinic by improving symptoms such as bradykinesia, rigidity and postural instability (4). Treatment of spinal cord injuries with GDNF has also produced neurological improvement.

The biological activity of Human GDNF was determined by its ability to support the survival and stimulate neurite outgrowth of cultured embryonic chick dorsal root ganglia neurons (Davies, A.M., 1989, Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor, Rush, R.A., ed., John Willey and Sons, Ltd., p.95). The expected ED₅₀ for this effect is typically 1 - 3 ng/ml.

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Lyophilized

The predicted molecular weight of Recombinant Human GDNF is Mr 30 kDa.

30

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Collins, F. et al. (1993) Science 260:1130 2. Ron, D. et al. (2008) Proc. Natl. Acad. Sci. (USA) 105:8114 3. Wang, C. Y. et al. (1998) Neuroscience 83:7 4. Kirik, D. et al. (2004) Nature Neuro. 7:105