Recombinant Human IGF-BP7

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Pricing & Details

Product. No.I-335
Alternate Names
Insulin-Like Growth Factor Binding Protein 7, IBP-7, Mac25, IGF Binding Protein Related Protein-1 [IGFBPrP1], FSTL2, IGFBP-7, IGFBP-7v, PSF
Expression Host
NS0 cells
Species
Human
Prod No.
Size
Price
Avail.
Qty
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I-335-25 µg
25 µg
$369.00
In stock
Max:
Min: 1
Step: 1

Data

Background

Insulin-like growth factor binding protein 7 (IGFBP7), also known as Mac25 and IGFBP-rp1, is a secretory protein. IGFBP7 is expressed in a wide range of normal human tissues with very high levels in HEVs of blood vessels in the secondary lymphoid tissues. IGFBP7 suppresses growth and colony formation of prostate and breast cancer cell lines through an IGF independent mechanism, which causes a delay in the G1 phase of the cell cycle and increased apoptosis. (1-2). The expression of IGFBP7 is upregulated in senescing epithelial cells and by retinoic acid (3). It plays a role in skeletal myogenesis by binding to IGF in a manner that inhibits IGF induced differentiation of skeletal myoblasts, without affecting IGF induced proliferation. IGFBP7 also interacts with heparin sulfate proteoglycans, type IV collagen and specific chemokines. It has also been implicated in angiogenisis.

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IGF-BP7 was determined by by its ability to bind rh6Ckine/CCL21. Immobilized rhIGFBP-rp1 at 1 μg/ml (100 μl/well) can bind rh6Ckine/CCL21 with a linear range of 0.08 - 5 ng/ml in a functional ELISA. (Nagakubo, D. et al., 2003, J.Immunol. 171:553).
Fusion Protein Tag
Histidine Tag
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
hhhhhhhhh dtcgpcepas cpplpplgcl lgetrdacgc cpmcargege pcggggagrg ycapgmecvk srkrrkgkag aaaggpgvsg vcvcksrypv cgsdgttyps gcqlraasqr aesrgekait qvskgtceqg psivtppkdi wnvtgaqvyl scevigiptp vliwnkvkrg hygvqrtell pgdrdnlaiq trggpekhev tgwvlvspls kedageyech asnsqgqasa sakitvvdal heipvkkgeg ael
N-terminal Sequence Analysis
His
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IGF-BP7 is Mr 27.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 36 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Tamura, K. et al. (2008) Reprod. Biol. Endocrinol. 6:54
2. Lai, M. et al. (2007) Cancer Biol. Ther. 6:354
3. Swisshelm, K. et al. (1995) Proc. Nat. Acad. Sci. (USA) 92:4474
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
I-335
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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