Recombinant Human IL-1 RII

Interleukin 1 receptor type II (IL1R2), also known as CD121b, is a transmembrane protein and member of the IL-1 receptor family. It is found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Like IL1R1, IL1R2 can bind all three forms of IL-1 (IL-1α, IL-1β and IL-1RA) but does not transmit IL-1 signals.¹ In addition to the membrane-bound form, IL1R2 also exists as the soluble form and either acts as a decoy receptor that inhibits IL-1 action by blocking the binding of IL-1 to the type I receptor complex.² Furthermore, IL-4 is reported to antagonize the activity of IL-1 by inducing the expression and release of IL1R2. Recombinant soluble IL1R2 is thus a potent antagonist of IL-1 action and serves as a potential therapeutic target.³ IL1R2 is involved in the control of the pro-inflammatory state that takes place in the endometrium during the premenstrual and menstrual periods. IL1R2 can neutralize IL-1β and counteract its effect on endometrial stromal cells, and therefore may provide a new clinical strategy for the treatment of endometriosis.⁴

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Phe14

Lyophilized

The predicted molecular weight of Recombinant Human IL-1 RII is Mr 36 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 40 kDa.

36

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. McMahan, CJ. et al. (1991) Embo. J. 10:2821
2. Colotta, F. et al. (1993) Science 261:472
3. Neumann, D. et al. (2000) J. Immunol. 165:3350
4. Akoum, A. et al. (2005) Hum. Rep. 20:1177