Recombinant Human IL-10 Rα

Interleukin 10 receptor, alpha subunit (IL-10 Rα), also known as IL10RA and CDw210a, is a subunit for the interleukin-10 receptor (IL-10 R). IL-10 Rα has been shown to mediate the immunosuppressive signal of IL-10 (1). IL-10 is a pleiotropic cytokine that regulates multiple immune responses through actions on T cells, B cells, macrophage/monocytes and antigen presenting cells (APC) and generally skews the immune response from TH1 to TH2. It may suppress immune responses by inhibiting expression of IL-1a, IL-1b, IL-6, IL-8, TNF-a, GM-CSF and G-CSF in activated monocytes and activated macrophages. IL-10 also suppresses IFN-γ production by NK cells. The biological effects of IL-10 are mediated through binding to the IL-10 receptor complex, which belongs to the class II cytokine receptor family (2). The IL-10 R complex is a heterodimer comprising of IL-10 Rα and IL-10 Rβ, which is shared by cytokine receptors for IL-22, IL-28 and IL-29. The IL-10 R is expressed on the majority of leukocytes including T cells, NK cells, macrophage/monocytes, B cells, neutrophils and dendritic cells. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway (3). Activation of IL-10 R also leads to tyrosine phosphorylation of JAK1 and TYK2 kinases (4).

The biological activity of Human IL-10 Rα was determined by its ability to inhibit IL-10 dependent proliferation of MC/9-2 mouse mast cells. The expected ED₅₀=0.05-0.25 µg/ml in the presence of 2 ng/ml or rHuman IL-10.

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His22

Lyophilized

The predicted molecular weight of Recombinant Human IL-10 Rα is Mr 24.4 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 25-35 kDa.

24

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Ho, A. et al. (1993) Proc. Natl. Acad. Sci. (USA) 90:11267
2. Josephson, K. et al. (2001) Immunity 15:35
3. Zhou, JH. et al. (2001) J. Immunol. 167:4436
4. Usacheva, A. et al. (2002) J. Immunol. 169:1302