Anti-Human IL-10 Rα

Purified Recombinant Human IL-10 Rα (Accession # Q13651.1)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

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Neutralization: This antibody is useful for neutralization of Human IL-10 Rα bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-10 Rα (at 0.25 ng/ml) is 0.03 - 0.1 µg/ml.

Mouse Anti-Human IL-10 Rα (Clone 37607) recognizes an epitope on Human IL-10 Rα. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Interleukin 10 receptor, alpha subunit is a subunit for the interleukin-10 receptor. IL10RA has also recently been designated CDW210A (cluster of differentiation W210A). It is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases.