Recombinant Human IL-17B

Interleukin-17B, also known as IL-17B, Cytokine CX1 and NIRF, belongs to the IL-17 family of structurally-related cytokines that share a highly conserved C-terminal region, but differ from one another in their N-terminal regions and in their distinct biological roles. IL-17B is expressed by T cells and stimulates the release of TNF-alpha and IL1-beta in the monocytic cell line, THP-1. It does not induce expression of IL1-alpha, IL-6, IFN-gamma or G-CSF in these cells (1-2). IL-17B is known to stimulate the proliferation of chondrocytes, bone and is highly expressed in nervous tissue, resulting in repair of diseased tissue. It is expressed highly in spinal cord and at lower levels in brain, pancreas, small intestine, lung and stomach (1). Expression of the IL-17B receptor (Evi27) appears to be restricted to human kidney, pancreas, liver, brain, and intestines. IL-17B, either alone or in combination with other cytokines or growth factors is known to aid wound healing (3).

The biological activity of Human IL-17B was determined by its ability to bind with rhIL-17B R/Fc in a functional ELISA.

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Met

Lyophilized

The predicted molecular weight of Recombinant Human IL-17B is Mr 18.3 kDa.

18.3

This recombinant protein was lyophilized from a 0.2 μm filtered solution in sodium dihydrogen phosphate (NaH₂PO₄) and sodium chloride (NaCl).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Li, H. et al. (2000) Proc. Nat. Acad. Sci. (USA) 97:773
2. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167
3. Kokubu, T. et al. (2008) J. Histochem. Cytochem. 56:89