Recombinant Human IL-17F

Recombinant Human IL-17F

Product No.: I-200

[product_table name="All Top" skus="I-200"]

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Alternate Names
ML1, LOC112744
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Human

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Select Product Size
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Background

IL-17F is a homodimeric protein that is a member of the IL17 family of cytokines produced by activated T-cells and monocytes.1 IL-17F is expressed by activated T cells and can stimulate production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover.2 IL-17F is an important regulator of inflammatory responses that function differently than IL-17 in immune responses and diseases.3

Protein Details

Purity
>97% by SDS Page and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-17F was determined by its ability to bind mIL-17 RC in a functional ELISA with an estimated KD < 3 nM.
Protein Accession No.
Amino Acid Sequence
The DNA sequence encoding the mature mouse IL-17F (Arg 21 - Ala 153) was inserted into a suitable expression vector and was expressed in E. coli (Hurst, S.D. et al., 2002, J. Immunol. 169(1):443 - 453).
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IL-17F is Mr 15 kDa.
Predicted Molecular Mass
15
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Starnes T. et al. (2001) J. of Immonol. 167: 4137 2. Starovasnik MA. et al. (2001) Embo J. 20: 5332 3. Chen Dong et al. (2008) J Experimental Med. 205: 1063

Certificate of Analysis

IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.