Recombinant Human IL-4 Receptor Alpha (IL-4 Rα)

Recombinant Human IL-4 Receptor Alpha (IL-4 Rα)

Product No.: I-345

[product_table name="All Top" skus="I-345"]

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Prod. No.I-345
Expression Host
sf Insect Cells

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IL-4 receptor alpha (IL-4 Rα), also known as IL4RA and CD124, is the alpha chain of the Il-4 receptor which binds to both IL-4 and IL-13 to regulate immune and allergenic responses. Hematopoietic cellular responses to IL-4 are mediated by a high affinity receptor complex comprised of the IL-4 Rα subunit and the common cytokine γ chain (CD132) (1). In non-hematopoietic cells, the IL-4 Rα subunit associates with the IL-13 Rα chain and serves as a functional complex for IL-13. IL-4 Rα is a single-pass type I transmembrane protein and similar to those present in the IL-2 receptor and GM-CSF receptor ß-chains. As a receptor for both IL-4 and IL-13, IL4 Rα couples to the JAK1/2/3-STAT6 pathway, and is involved in regulating IgE production and chemokine and mucus production at sites of allergic inflammation, as well as promoting differentiation of Th2 cells (2). The secreted extracellular domain of IL4 Rα, called sIL-4 Rα, can inhibit IL-4-mediated cell proliferation and IL-5 up-regulation by T cells but it has no signaling abilities. Allelic variations in the IL-4 Rα gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitis, asthma or eczema (3).

Protein Details

>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Soluble Interleukin-4 Receptor α is determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells. The expected ED<sub>50</sub> for this effect is 2 x 10<sup>5</sup> units/mg.
Amino Acid Sequence
gnmkvlq eptcvsdyms istcewkmng ptncstelrl lyqlvfllse ahtcipenng gagcvchllm ddvvsadnyt ldlwagqqll wkgsfkpseh vkprapgnlt vhtnvsdtll ltwsnpyppd nylynhltya vniwsendpa dfriynvtyl epslriaast lksgisyrar vrawaqcynt twsewspstk whnsyrepfe qh
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IL-4R Alpha is Mr 24 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 30-35 kDa.
Predicted Molecular Mass
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701
2. Jiang, H. et al. (2000) J. Allergy Clin. Immunol. 105:1063
3. Hall, IP. et al. (2000) Respir. Res. 1:6
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.