Recombinant Human Latent TGF-β1

Transforming growth factor beta 1 or TGF-β1 is a multifunctional cytokine that regulates the proliferation and differentiation of cells specific to bone like chondrocytes, osteoblasts, osteoclasts including mesenchymal precursor cells.¹ It is known to promote the pathogenesis of lung fibrosis and acts as a tumor suppressor in normal cells.² TGF-beta1 can promote mechanical strength in healing Achilles tendons by regulating collagen synthesis, cross-link formation, and matrix remodeling.³

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Leu30 & Ala279

Solution

The predicted molecular weight of Recombinant Human TGF-β1 is 28.5 kDa (LAP) & 12.8 kDa (Mature), monomer. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 80-95 kDa & 24 kDa under nonreducing conditions, and 36-42 kDa & 11 kDa under reducing conditions.

41.3

This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) and glycerol pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.

Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.

Dry Ice

1. Guven, M. et al. (2007) Growth Factors 25: 101
2. Schneider, J. et al. (2009) J Investing Med. 57(5):655-61
3. Yu, C. et al. (2009) Biochem Biophys Res Commun. 406(2):204-210