Recombinant Human MCP-3

Monocyte chemotactic protein-3 (MCP3) is a non-glycosylated polypeptide belonging to the the CC-Chemokines family that attracts monocytes and eosinophils, augments monocyte anti-tumor activity, and induces the release of gelatinase B. It also regulates protease secretion by macrophages. MCP3 can bind heparin.¹ MCP-3 is one of the most pluripotent chemokines since it activates all types of leukocytes.
MCP-3 may affect HIV-1 infection via inhibiting the binding of HIV envelope to CCR5.² MCP-3 has also been implicated in Multiple Sclerosis, allergic reactions and viral infections.^3,4,5

The biological activity of Human MCP-3 was determined by by its ability to chemoattract 2 day cultured human monocytes (Matsushima, K. et al., 1989, J. Exp. Med. 169:1485 - 1490) and its ability to chemoattract mouse BaF/3 cells transfected with hCCR2A. The expected ED₅₀ for these effects are typically 0.02 - 0.08 μg/ml and 0.1 - 0.5 μg/ml, respectively.

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Gln34

Lyophilized

The predicted molecular weight of Recombinant Human MCP-3 is Mr 9 kDa.

9

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final antibody concentration of 100 ug/mL

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Van Damme, J. et al. (1993) Biochem. Biophys. Res. Commun. 191: 535
2. Parmentier, M. et al. (1999) Blood 94: 1899
3. Brosnan, CF. et al. (1998) J Neuroimmunol. 86: 20
4. Reddigari, SR. et al. (1995) Exp. Dermatol. 4: 260
5. Matikainen, S. et al. (2001) Cytokine Growth Factor Rev. 12: 171