Recombinant Human MCP-3

Product No.: M158


Alternate Names CCL7, SCYA6, SCYA7, Small Inducible Cytokine A7, CCL7, NC28, Chemokine (C-C Motif) Ligand 7, FIC, MARC, MGC138463, MGC138465 Product Type Recombinant Protein Expression Host E. coli Cells Species Human


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Background Monocyte chemotactic protein-3 (MCP3) is a non-glycosylated polypeptide belonging to the the CC-Chemokines family that attracts monocytes and eosinophils, augments monocyte anti-tumor activity, and induces the release of gelatinase B. It also regulates protease secretion by macrophages. MCP3 can bind heparin.¹ MCP-3 is one of the most pluripotent chemokines since it activates all types of leukocytes. MCP-3 may affect HIV-1 infection via inhibiting the binding of HIV envelope to CCR5.² MCP-3 has also been implicated in Multiple Sclerosis, allergic reactions and viral infections.^3,4,5 Protein Details Purity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.01 EU/µg as determined by the LAL method Biological Activity The biological activity of Human MCP-3 was determined by by its ability to chemoattract 2 day cultured human monocytes (Matsushima, K. et al., 1989, J. Exp. Med. 169:1485 - 1490) and its ability to chemoattract mouse BaF/3 cells transfected with hCCR2A. The expected ED<sub>50</sub> for these effects are typically 0.02 - 0.08 μg/ml and 0.1 - 0.5 μg/ml, respectively. Protein Accession No. Q7Z7Q8 Amino Acid Sequence qpvgint sttccyrfin kkipkqrles yrrttsshcp reavifktkl dkeicadptq kwvqdfmkhl dkktqtpkl N-terminal Sequence Analysis Gln34 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human MCP-3 is Mr 9 kDa. Predicted Molecular Mass 9 Formulation This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA). Reconstitution This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final antibody concentration of 100 ug/mL Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank 6354 References & Citations 1. Van Damme, J. et al. (1993) Biochem. Biophys. Res. Commun. 191: 535 2. Parmentier, M. et al. (1999) Blood 94: 1899 3. Brosnan, CF. et al. (1998) J Neuroimmunol. 86: 20 4. Reddigari, SR. et al. (1995) Exp. Dermatol. 4: 260 5. Matikainen, S. et al. (2001) Cytokine Growth Factor Rev. 12: 171 View product citations for antibody PRODUCT_CODE on CiteAb Certificate of Analysis Request COA IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.

Background

Monocyte chemotactic protein-3 (MCP3) is a non-glycosylated polypeptide belonging to the the CC-Chemokines family that attracts monocytes and eosinophils, augments monocyte anti-tumor activity, and induces the release of gelatinase B. It also regulates protease secretion by macrophages. MCP3 can bind heparin.¹ MCP-3 is one of the most pluripotent chemokines since it activates all types of leukocytes.
MCP-3 may affect HIV-1 infection via inhibiting the binding of HIV envelope to CCR5.² MCP-3 has also been implicated in Multiple Sclerosis, allergic reactions and viral infections.^3,4,5

Protein Details

Purity

>97% by SDS-PAGE and analyzed by silver stain.

Endotoxin Level

<0.01 EU/µg as determined by the LAL method

Biological Activity

The biological activity of Human MCP-3 was determined by by its ability to chemoattract 2 day cultured human monocytes (Matsushima, K. et al., 1989, J. Exp. Med. 169:1485 - 1490) and its ability to chemoattract mouse BaF/3 cells transfected with hCCR2A. The expected ED<sub>50</sub> for these effects are typically 0.02 - 0.08 μg/ml and 0.1 - 0.5 μg/ml, respectively.

Protein Accession No.

Q7Z7Q8

Amino Acid Sequence

qpvgint sttccyrfin kkipkqrles yrrttsshcp reavifktkl dkeicadptq kwvqdfmkhl dkktqtpkl

N-terminal Sequence Analysis

Gln34

State of Matter

Lyophilized

Predicted Molecular Mass

The predicted molecular weight of Recombinant Human MCP-3 is Mr 9 kDa.

Predicted Molecular Mass

Formulation

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

Reconstitution

This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final antibody concentration of 100 ug/mL

Storage and Stability

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Country of Origin

USA

Shipping

Next Day Ambient

NCBI Gene Bank

6354

References & Citations

1. Van Damme, J. et al. (1993) Biochem. Biophys. Res. Commun. 191: 535
2. Parmentier, M. et al. (1999) Blood 94: 1899
3. Brosnan, CF. et al. (1998) J Neuroimmunol. 86: 20
4. Reddigari, SR. et al. (1995) Exp. Dermatol. 4: 260
5. Matikainen, S. et al. (2001) Cytokine Growth Factor Rev. 12: 171

View product citations for antibody PRODUCT_CODE on CiteAb

Certificate of Analysis

Request COA

IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.

Prod No.	Description
M153	Anti-Human MCP-3 (Clone 36320)
M144	Anti-Human MCP-3
M158	Recombinant Human MCP-3
M175	Anti-Mouse CCL7
M134	Recombinant Mouse MCP-3
M126	Anti-Mouse MCP-3 – Biotin
M125	Anti-Human MCP-3 – Biotin

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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