Recombinant Human MIA

Melanoma inhibitory activity (MIA), also known as cartilage derived retinoic acid sensitive protein (CD-RAP)¹, is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin. MIA is over-expressed in pancreatic cancer and has the potential of promoting the invasiveness of pancreatic cancer cells.²

The biological activity of Human Melanoma Inhibitory Activity is determined by the dose-dependent proliferation of the human A375 melanoma cell line. The expected ED₅₀ for this effect is 4-6 mg/ml.

MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ

Met, Gly25

Lyophilized

The predicted molecular weight of Recombinant Human MIA is Mr 12 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 10 kDa.

12

This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution in PBS, pH 7.4 with 5% Trehalose.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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1. Sandell, LJ. et al. (1996) J Biol Chem. 271: 3311
2. Friess, H. et al. (2005) Cancer Cell Int. 5: 3