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BackgroundMelanoma inhibitory activity (MIA), also known as cartilage derived retinoic acid sensitive protein (CD-RAP)1, is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin. MIA is over-expressed in pancreatic cancer and has the potential of promoting the invasiveness of pancreatic cancer cells.2 Protein DetailsPurity >95% by SDS-PAGE and HPLC Endotoxin Level <0.1 EU/µg as determined by the LAL method Biological Activity The biological activity of Human Melanoma Inhibitory Activity is determined by the dose-dependent proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 mg/ml. Expression Host E. coli Protein Accession No. Amino Acid Sequence MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ N-terminal Sequence Analysis Met, Gly25 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human MIA is Mr 12 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 10 kDa. Formulation This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution in PBS, pH 7.4 with 5% Trehalose. Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day Ambient PubMed NCBI Gene Bank References & Citations1. Sandell, LJ. et al. (1996) J Biol Chem. 271: 3311 2. Friess, H. et al. (2005) Cancer Cell Int. 5: 3 IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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