Recombinant Human MIA

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Pricing & Details

Product. No.M109
Alternate Names
Cartilage-Derived Retinoic Acid-Sensitive Protein [CD-RAP]
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
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M109-100 μg
100 µg
$339.00
In stock
Max:
Min: 1
Step: 1

Data

Background

Melanoma inhibitory activity (MIA), also known as cartilage derived retinoic acid sensitive protein (CD-RAP)1, is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin. MIA is over-expressed in pancreatic cancer and has the potential of promoting the invasiveness of pancreatic cancer cells.2

Protein Details

Purity
>95% by SDS-PAGE and HPLC
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Melanoma Inhibitory Activity is determined by the dose-dependent proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 mg/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ
N-terminal Sequence Analysis
Met, Gly25
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human MIA is Mr 12 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 10 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution in PBS, pH 7.4 with 5% Trehalose.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Sandell, LJ. et al. (1996) J Biol Chem. 271: 3311
2. Friess, H. et al. (2005) Cancer Cell Int. 5: 3
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
M109
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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