Recombinant Human Pleiotrophin

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Pricing & Details

Product. No.P119
Alternate Names
PTN, HARP, Heparin Binding Growth Factor 8 (HBGF8), HBNF, Neurite Growth-Promoting Factor 1 (NEGF1)
Expression Host
Sf 21 Insect Cells
Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
P119-50 µg
50 µg
$309.00
In stock
Max:
Min: 1
Step: 1
P119-250 µg
250 µg
$889.00
In stock
Max:
Min: 1
Step: 1

Background

Pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1), also known as PTN is a growth, differentiation inducing cytokine,1 and neurite extension promoting polypeptide which is highly expressed in brain and in tissues derived from mesenchyme.2 PTN is produced by many different human cancers and stimulates tumor blood vessel formation when it is expressed in malignant cancer cells.3 It is expressed in many types of cancer, such as gliomas, melanomas, meningiomas, neuroblastomas, choriocarcinomas, leukemias and cancers of pancreas, prostate, stomach, colon, breast, ovaries and lungs.4

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human PTN was determined by its ability to enhance neurite outgrowth of cerebral cortical neurons of E10 chick embryos (Hampton, B.S. et al., 1992, Mol. Biol. Cell 3:85 - 93). Optimal neurite outgrowth was observed when neurons were plated on 96 well culture plates that had been pre-coated with 100 μL/well of a solution of 3 - 8 μg/ml rhPTN.
Expression Host
Sf 21 Insect Cells
Protein Accession No.
Q6ICQ5
Amino Acid Sequence
Gil33-Asp168
N-terminal Sequence Analysis
Gly33
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human PTN is Mr 15.3 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 18 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Lee, RJ. et al. (2005) Biochem Biophys Res Commun. 332: 1146
2. Deuel, TF. et al. (1992) Biochem Biophys Res Commun. 184: 427
3. Berenson, JR. et al. (2009) Blood 113: 1992
4. Papadimitriou, E. et al. (2007) Recent Patents Anticancer Drug Discov. 2: 175
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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