Recombinant Human Prolactin Receptor

Prolactin Receptor (PRL-R) is a cytokine receptor that lacks an intrinsic kinase domain and relies on the cytoplasmic Jak tyrosine kinases to transduce signals (1). PRL-R is required for the signaling and proliferation of prolactin (2). Prolactin receptors have been localized in a number of peripheral tissues, including tissues involved in reproduction (3). Within the immune system, multiple isoforms of PRL-R serve to mediate the effects of prolactin. These isoforms are structurally and functionally distinct, causing significant differences in ligand affinities, kinetics of transduction and the transduction proteins activated. Differential activation of these pathways may contribute to the pleiotropism of prolactin action in tissues of the immune system (4). PRL-R is required for normal maternal glucose homeostasis during pregnancy (5).

The biological activity of Human PRL-R was determined by its ability to neutralize rhProlactin-induced proliferation of Nb2-11 cells (a rat lymphoma cell line) (Guot, P.W. et al., 1980, Cancer Research 40:2433). The expected ED₅₀ of this effect is typically 0.15-0.75 μg/ml in the presence of 0.5 ng/ml of Recombinant Human Prolactin.

Fc Fusion Protein

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Gln25 predicted

Lyophilized

The predicted molecular weight of Recombinant Human PRL-R is Mr 51 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 66 kDa.

51

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Lindeman, GJ. et al. (2003) J. Biol. Chem. 278:32929
2. Clevenger, V. et al. (1998) Mol. Cell Biol. 8:896
3. Makrigiannakis, A. et al. (2005) Reproductive Biomed. 10:339
4. Clevenger, CV. et al. (2001) Lupus 10:706
5. Mastro, M. et al. (2003) J. Appl. Physiol. 94:518