Recombinant Human TGF-α

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Pricing & Details

Product. No.T151
Alternate Names
Transforming Growth Factor-Alpha, Sacroma Growth Factor, TGF-Type I, ETGF, MDGF-2 (Milk-Derived Growth Factor-2), TCGF (Transformed Cell Growth Factor)
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
T151-100 µg
100 µg
$339.00
In stock
Max:
Min: 1
Step: 1
T151-500 µg
500 µg
$1,049.00
In stock
Max:
Min: 1
Step: 1

Background

Transforming growth factor alpha (TGF-α), also known as TCGF and MDGF-2, is an acid- and heat-stable protein and member of the EGF family of cytokines. It is thought to be the major autocrine factor controlling growth in epidermal cells (1). TGF-α plays an important role in cell proliferation during embryogenesis and stimulates neural cell proliferation in the adult injured brain (2). It is produced by monocytes, keratinocytes, and various tumor cells. TGF-α binds to the EGF receptor, mediating tyrosine phosphorylation of the receptor, and promotes cell-cell adhesion and to cell-cell stimulation (1). It is probably involved in the regeneration of liver tissues (3) and also affects bone formation/remodeling by inhibition of the synthesis of collagen and release of calcium. It has been suggested that TGF-α may act as an autocrine growth factor for the induction or maintenance of malignancy. It is present in high levels in around half of all breast tumors, and these tumors tend to be more aggressive and more likely to spread to the lungs (4). It has been shown that TGF-α can prompt breast cancer cells to make another cytokine called angiopoietin-like 4 (ANGPTL4), which helps the cells to spread through the bloodstream (5). Human, murine and rat TGF-α are cross-species reactive.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Transforming Growth Factor-Alpha is determined by determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells. The expected ED50 for this effect is 0.2 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
v vshfndcpds htqfcfhgtc rflvqedkpa cvchsgyvga rcehadlla
N-terminal Sequence Analysis
Val40
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human TGF-α is Mr 6 kDa.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution and addition of a carrier protein such as 0.1% BSA or HSA, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C for three months in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Anklesaria, P. et al. (1990) Proc. Natl. Acad. Sci. (USA) 87:3289
2. Loughlin, S. et al. (2002) Proc. Natl. Acad. Sci. (USA) 97:14686
3. Mead, JE. et al. (1989) Proc. Natl. Acad. Sci. (USA) 86:1558
4. Ciardiello, F. et al. (1991) Ann. Oncol. 2:169
5. Minn, AJ. et al. (2005) Nature 436:518
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
T145
T151
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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