Recombinant Human TNF RI

Tumor necrosis factor receptor superfamily, member 1A, (TNFRI) also known as TNFRSF1A or CD120A is the principal receptor through which many of the pro-inflammatory activities of TNFα are mediated.² TNFRI contains a death domain through which it interacts with other death-domain proteins to promote cellular responses.² TNFRI is found in both membrane-bound and soluble forms. It interacts with membrane-bound or soluble forms of its ligand (TNFα), respectively. Binding of membrane-bound TNFα to the membrane-bound receptor is involved in cell survival, apoptosis, and inflammation by inducing receptor trimerization and activation. ⁴ Proteolytic processing of TNFRI yields the release of the soluble form of the receptor (TNFsRI), which inhibits inflammation via the interaction with free TNFα.

The biological activity of Human TNF sRI was determined by measuring its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The expected ED₅₀ for this effect is approximately 0.4 - 1.0 ng/ml in the presence of 0.25 ng/ml rhTNF-α¹.

Fc Fusion Protein

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Leu30 & Asp41

Lyophilized

The predicted molecular weight of monomeric Recombinant Human TNF sRI is Mr 48 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE under reducing conditions is Mr 60-66 kDa. TNF sRI is expressed in NSO cells as a disulfide-linked homodimer.

48

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Matthews, N. and Neale, ML. (1987). Lymphokines and Interferons, A Practical Approach 221-225.
2. Dana, R. et al. (2000) Arch Ophthalmol. 118: 1666
3. Donner, DB. et al. (2008) J Immunol. 181: 1288
4. Pasparakis, M. et al. (2008) Nat Immunol. 9: 1015