Recombinant Human TNF RI

Recombinant Human TNF RI

Product No.: T390

[product_table name="All Top" skus="T390"]

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Alternate Names
TNFRSF1A, P55, TBP1, CD120a, FPF, MGC19588, TNF-R, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55-R, p60
Product Type
Recombinant Protein
Expression Host
NS0 Cells
Species
Human

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Background

Tumor necrosis factor receptor superfamily, member 1A, (TNFRI) also known as TNFRSF1A or CD120A is the principal receptor through which many of the pro-inflammatory activities of TNFα are mediated.2 TNFRI contains a death domain through which it interacts with other death-domain proteins to promote cellular responses.2 TNFRI is found in both membrane-bound and soluble forms. It interacts with membrane-bound or soluble forms of its ligand (TNFα), respectively. Binding of membrane-bound TNFα to the membrane-bound receptor is involved in cell survival, apoptosis, and inflammation by inducing receptor trimerization and activation. 4 Proteolytic processing of TNFRI yields the release of the soluble form of the receptor (TNFsRI), which inhibits inflammation via the interaction with free TNFα.

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human TNF sRI was determined by measuring its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The expected ED<sub>50</sub> for this effect is approximately 0.4 - 1.0 ng/ml in the presence of 0.25 ng/ml rhTNF-α<sup>1</sup>.
Fusion Protein Tag
Fc Fusion Protein
Protein Accession No.
Amino Acid Sequence
(lvphlgdrekr) dsvcpqgkyi hpqnnsicct kchkgtylyn dcpgpgqdtd crecesgsft asenhlrhcl scskcrkemg qveissctvd rdtvcgcrkn qyrhywsenl fqcfncslcl ngtvhlscqe kqntvctcha gfflrenecv scsnckksle ctklclpqie vkgtedsgtt niegrmdksc dkthtcppcp apellggpsv flfppkpkdt lmisrtpevt cvvvdvshed pevkfnwyvd gvevhnaktk preeqynsty rvvsvltvlh qdwlngkeyk ckvsnkalpa piektiskak gqprepqvyt lppsrdeltk nqvsltclvk gfypsdiave wesngqpenn ykttppvlds dgsfflyskl tvdksrwqqg nvfscsvmhe alhnhytqks lslspgkhhh hhh
N-terminal Sequence Analysis
Leu30 & Asp41
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of monomeric Recombinant Human TNF sRI is Mr 48 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE under reducing conditions is Mr 60-66 kDa. TNF sRI is expressed in NSO cells as a disulfide-linked homodimer.
Predicted Molecular Mass
48
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Matthews, N. and Neale, ML. (1987). Lymphokines and Interferons, A Practical Approach 221-225.
2. Dana, R. et al. (2000) Arch Ophthalmol. 118: 1666
3. Donner, DB. et al. (2008) J Immunol. 181: 1288
4. Pasparakis, M. et al. (2008) Nat Immunol. 9: 1015

Certificate of Analysis

IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.