Recombinant Human VEGF 165 Protein
Recombinant Human VEGF 165 Protein
Product No.: V101
Product. No.V101 Alternate Names Vascular Endothelial Growth Factor 165, VEGFA, MGC70609, VPF, VAS, Folliculostellate Cell-Derived Growth Factor, Glioma-Derived Endothelial Cell Mitogen Product Type Recombinant Protein Expression Host sf Insect Cells Species Human |
Data
BackgroundVascular endothelial growth factor (VEGF), a potent proangiogenic cytokine1 is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.3,4,5,6 Protein DetailsPurity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.01EU/µg as determined by the LAL method Biological Activity The biological activity of Human VEGF was determined by a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The expected ED50 is typically 2.0-6.0 ng/ml. Protein Accession No. Amino Acid Sequence apma egggqnhhev vkfmdvyqrs ychpietlvd ifqeypdeie yifkpscvpl mrcggccnde glecvptees nitmqimrik phqgqhigem sflqhnkcec rpkkdrarqe npcgpcserr khlfvqdpqt ckcsckntds rckarqleln ertcrcdkpr r N-terminal Sequence Analysis Ala27 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human VEGF is Mr 19.2 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 20-22 kDa (reducing conditions) and
39-42 kDa (non-reducing conditions). Formulation This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA). Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient PubMed References & Citations1. Folkman, J. et al. (2008) FASEB J. 22: 3728 2. Goodsell, DS. et al. (2002) The Oncologist 7: 569 3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13: 130 4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312: 549 5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28: 488 6. Ellis, LM. et al. (2005) J Clin Oncol. 23: 1011 IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.