Recombinant Human VEGF 165 Protein

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Pricing & Details

Prod. No.V101
Expression Host
Sf 21 Insect Cells
Size
Price
Add to cart
10 µg
299.00
Max:
Min: 1
Step: 1
50 µg
969.00
Max:
Min: 1
Step: 1
500 µg
2,405.00
Max:
Min: 1
Step: 1

Data

Background

Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine1 is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.3,4,5,6

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human VEGF was determined by a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The expected ED50 is typically 2.0-6.0 ng/ml.
Expression Host
Sf 21 Insect Cells
Protein Accession No.
Amino Acid Sequence
apma egggqnhhev vkfmdvyqrs ychpietlvd ifqeypdeie yifkpscvpl mrcggccnde glecvptees nitmqimrik phqgqhigem sflqhnkcec rpkkdrarqe npcgpcserr khlfvqdpqt ckcsckntds rckarqleln ertcrcdkpr r
N-terminal Sequence Analysis
Ala27
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human VEGF is Mr 19.2 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 20-22 kDa (reducing conditions) and 39-42 kDa (non-reducing conditions).
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed

References & Citations

1. Folkman, J. et al. (2008) FASEB J. 22: 3728
2. Goodsell, DS. et al. (2002) The Oncologist 7: 569
3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13: 130
4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312: 549
5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28: 488
6. Ellis, LM. et al. (2005) J Clin Oncol. 23: 1011
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
V101
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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