Recombinant Human VEGF 165 Protein

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Recombinant Human VEGF 165 Protein

Product No.: V101

[product_table name="All Top" skus="V101"]

Product. No.V101
Alternate Names
Vascular Endothelial Growth Factor 165, VEGFA, MGC70609, VPF, VAS, Folliculostellate Cell-Derived Growth Factor, Glioma-Derived Endothelial Cell Mitogen
Product Type
Recombinant Protein
Expression Host
sf Insect Cells

Product Size



Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine1 is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.3,4,5,6

Protein Details

>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human VEGF was determined by a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The expected ED50 is typically 2.0-6.0 ng/ml.
Protein Accession No.
Amino Acid Sequence
apma egggqnhhev vkfmdvyqrs ychpietlvd ifqeypdeie yifkpscvpl mrcggccnde glecvptees nitmqimrik phqgqhigem sflqhnkcec rpkkdrarqe npcgpcserr khlfvqdpqt ckcsckntds rckarqleln ertcrcdkpr r
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human VEGF is Mr 19.2 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 20-22 kDa (reducing conditions) and 39-42 kDa (non-reducing conditions).
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient

References & Citations

1. Folkman, J. et al. (2008) FASEB J. 22: 3728
2. Goodsell, DS. et al. (2002) The Oncologist 7: 569
3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13: 130
4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312: 549
5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28: 488
6. Ellis, LM. et al. (2005) J Clin Oncol. 23: 1011
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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