Recombinant Human VEGF 165 Protein

Product No.: V101


Alternate Names Vascular Endothelial Growth Factor 165, VEGFA, MGC70609, VPF, VAS, Folliculostellate Cell-Derived Growth Factor, Glioma-Derived Endothelial Cell Mitogen Product Type Recombinant Protein Expression Host sf Insect Cells Species Human


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Background Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine¹ is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.² VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.^3,4,5,6 Protein Details Purity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.01EU/µg as determined by the LAL method Biological Activity The biological activity of Human VEGF was determined by a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The expected ED<sub>50</sub> is typically 2.0-6.0 ng/ml. Protein Accession No. NP_001165097 Amino Acid Sequence apma egggqnhhev vkfmdvyqrs ychpietlvd ifqeypdeie yifkpscvpl mrcggccnde glecvptees nitmqimrik phqgqhigem sflqhnkcec rpkkdrarqe npcgpcserr khlfvqdpqt ckcsckntds rckarqleln ertcrcdkpr r N-terminal Sequence Analysis Ala27 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human VEGF is Mr 19.2 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 20-22 kDa (reducing conditions) and 39-42 kDa (non-reducing conditions). Predicted Molecular Mass 19.2 Formulation This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA). Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient References & Citations 1. Folkman, J. et al. (2008) FASEB J. 22: 3728 2. Goodsell, DS. et al. (2002) The Oncologist 7: 569 3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13: 130 4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312: 549 5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28: 488 6. Ellis, LM. et al. (2005) J Clin Oncol. 23: 1011 View product citations for antibody PRODUCT_CODE on CiteAb Certificate of Analysis Request COA IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.

Background

Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine¹ is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.² VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.^3,4,5,6

Protein Details

Purity

>97% by SDS-PAGE and analyzed by silver stain.

Endotoxin Level

<0.01EU/µg as determined by the LAL method

Biological Activity

The biological activity of Human VEGF was determined by a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The expected ED<sub>50</sub> is typically 2.0-6.0 ng/ml.

Protein Accession No.

NP_001165097

Amino Acid Sequence

apma egggqnhhev vkfmdvyqrs ychpietlvd ifqeypdeie yifkpscvpl mrcggccnde glecvptees nitmqimrik phqgqhigem sflqhnkcec rpkkdrarqe npcgpcserr khlfvqdpqt ckcsckntds rckarqleln ertcrcdkpr r

N-terminal Sequence Analysis

Ala27

State of Matter

Lyophilized

Predicted Molecular Mass

The predicted molecular weight of Recombinant Human VEGF is Mr 19.2 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 20-22 kDa (reducing conditions) and 39-42 kDa (non-reducing conditions).

Predicted Molecular Mass

19.2

Formulation

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

Storage and Stability

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Country of Origin

USA

Shipping

Next Day Ambient

References & Citations

1. Folkman, J. et al. (2008) FASEB J. 22: 3728
2. Goodsell, DS. et al. (2002) The Oncologist 7: 569
3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13: 130
4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312: 549
5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28: 488
6. Ellis, LM. et al. (2005) J Clin Oncol. 23: 1011

View product citations for antibody PRODUCT_CODE on CiteAb

Certificate of Analysis

Request COA

IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.

Prod No.	Description
V101	Recombinant Human VEGF 165 Protein

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Recombinant Human VEGF 165 Protein