Recombinant Human VEGF-B

Recombinant Human VEGF-B

Product No.: V127

[product_table name="All Top" skus="V127"]

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Alternate Names
Vascular Endothelial Growth Factor-B, VEGFL, VRF
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Human

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Background

Vascular endothelial growth factor B (VEGF-B) is a member of the VEGF family and particularly abundant in heart and skeletal muscle.1 It is an angiogenic and neuroprotective protein that reduces hypoxic and ischemic neuronal injury.2 VEGF-B has a role in vascularization of the heart, skeletal muscles and developing bones, and in paracrine interactions between endothelial and surrounding muscle cells.3 VEGF-B also regulates lipid metabolism.4

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human VEGF-B was determined by its binding ability in a functional ELISA. Immobilized recombinant rat Neuropilin1 at 4 μg/ml (100 μl/well) can bind recombinant human VEGFB 167 with a linear range of 0.3-20 ng/ml.
Protein Accession No.
Amino Acid Sequence
pvsqpdapg hqrkvvswid vytratcqpr evvvpltvel mgtvakqlvp scvtvqrcgg ccpddglecv ptgqhqvrmq ilmirypssq lgemsleehs qcecrpkkkd savkpdsprp lcprctqhhq rpdprtcrcr crrrsflrcq grglelnpdt crcrklrr
N-terminal Sequence Analysis
Pro22
State of Matter
Solution
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human VEGF-B is Mr 19 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 19-22 kDa (reducing conditions).
Predicted Molecular Mass
19
Formulation
This recombinant protein was 0.2 μm filtered and is supplied as a solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
The protein should be stored at -20 - -70°C upon receipt. It may be stored at 2 - 8°C for one month. However, it is recommended to aliquot into working volumes and store at -20 - -70°C in a manual defrost freezer. Avoid repeat freeze thaw cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
NCBI Gene Bank

References & Citations

1. Eriksson, U. et al. (1993) Proc Natl Acad Sci USA 93: 2576
2. Greenberg, DA. et al. (2006) Dev Biol. 289: 329
3. Eriksson, U. et al. (1999) Dev Dyn. 215: 12
4. Alitalo, K. et al. (2008) Circ Res. 103: 1018

Certificate of Analysis

IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Prod No.
Description
V127
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.