WNT1 inducible signaling pathway protein 3, also known as WISP3 is a secreted tumor suppressor protein.1 It regulates the expression of cartilage-specific molecules that sustain chondrocyte growth and cartilage integrity. WISP-3 can function as a ligand and signal via autocrine and/or paracrine modes upon being secreted by chondrocytes.2 WISP3 is also essential for normal postnatal skeletal growth and cartilage homeostasis.3 IT is considered a novel target in development of Microsatellite instability (MSI-H) colorectal carcinomas.4
>98% by SDS Page and HPLC
The biological activity of Human WNT-1 Inducible Signaling Pathway Protein-3 is determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 0.2-0.3 µg/ml.
Amino Acid Sequence
TGPLDTTPEG RPGEVSDAPQ RKQFCHWPCK CPQQKPRCPP GVSLVRDGCG CCKICAKQPG EICNEADLCD PHKGLYCDYS VDRPRYETGV CAYLVAVGCE FNQVHYHNGQ VFQPNPLFSC LCVSGAIGCT PLFIPKLAGS HCSGAKGGKK SDQSNCSLEP LLQQLSTSYK TMPAYRNLPL IWKKKCLVQA TKWTPCSRTC GMGISNRVTN ENSNCEMRKE KRLCYIQPCD SNILKTIKIP KGKTCQPTFQ LSKAEKFVFS GCSSTQSYKP TFCGICLDKR CCIPNKSKMI TIQFDCPNEG SFKWKMLWIT SCVCQRNCRE PGDIFSELKI L
State of Matter
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
References & Citations
1. Kleer CG et al. (2004) Neoplasia 6: 179 2. Sen M et al. (2001) Gastroenterology 121: 1275 3. Hurvitz JR et al. (1999) Nature Genetics 23: 94 4. Lothe RA et al. (2008) Mol Cell Biol. 28: 1114
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.