Activated leukocyte cell adhesion molecule (ALCAM/CD166) is an 82 kDa transmembrane glycoprotein and a member of the immunoglobulin gene superfamily. It is also known as MEMD, SC-1/DM-GRASP and KG-CAM. It is linked with cell migration and development and with cancer progression (1). ALCAM is expressed on natural killer cells, thymocytes, platelets, B cells, T cells and monocytes after cell activation, as well as eosinophils, fibroblasts, endothelial cells, keratinocytes and neurons (2). It is a ligand for CD6 and NgCAM but also engages in homophilic interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation (3).
>95% by SDS-PAGE and analyzed by silver stain.
<0.1 EU/µg as determined by the LAL method
The biological activity of Mouse ALCAM was determined by its ability to block adhesion of HUT-78, a human cutaneous T cell lymphoma, to immobilized rhCD6/Fc protein. rmALCAM/Fc at 10 μg/mL (50 μL/well) blocked 100% of HUT-78 cell adhesion (1 x 106 cells/mL, 50 μL/well) to immobilized rhCD6/Fc (10 μg/mL, 100 μL/well).
Protein Accession No.
Amino Acid Sequence
wyt vnsaygdtiv mpcrldvpqn lmfgkwkyek pdgspvfiaf rsstkksvqy ddvpeykdrl slsenytlsi anakisdekr fvcmlvtedn vfeaptlvkv fkqpskpeiv nkapfletdq lkklgdcisr dsypdgnitw yrngkvlqpv egevailfkk eidpgtqlyt vtssleyktt rsdiqmpftc svtyygpsgq ktiyseqeif diyypteqvt iqvlppknai kegdnitlqc lgngnpppee fmfylpgqpe girssntytl tdvrrnatgd ykcslidkrn maasttitvh yldlslnprg evtkqigdtl pvsctisasr natvvwmkdn irlrsspsfs slhyqdagny vcetalqeve glkkresltl ivegkpqikm tkktdpsgls ktiichvegf pkpaihwtit gsgsvinqte espyingryy skiiispeen vtltctaenq lertvnslnv saisipehde addisdenre kvndqakieg rmdpkscdkt htcppcpape llggpsvflf ppkpkdtlmi srtpevtcvv vdvshedpev kfnwyvdgve vhnaktkpre eqynstyrvv svltvlhqdw lngkeykckv snkalpapie ktiskakgqp repqvytlpp srdetknqvs ltclvkgfyp sdiavewesn gqpennyktt ppvldsdgsf flyskltvdk srwqqgnvfs csvmhealhn hytqkslsls pgk
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse ALCAM is Mr 82 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 114-122 kDa.
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Weichert, W. et al. (2004) J. Clin. Pathol. 57:1160
2. Bowen, MA. et al. (1995) J. Exp. Med. 181:2213
3. Aruffo, A. et al. (1997) Immunol. Today 18:498
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.