Recombinant Mouse ALCAM

Activated leukocyte cell adhesion molecule (ALCAM/CD166) is an 82 kDa transmembrane glycoprotein and a member of the immunoglobulin gene superfamily. It is also known as MEMD, SC-1/DM-GRASP and KG-CAM. It is linked with cell migration and development and with cancer progression (1). ALCAM is expressed on natural killer cells, thymocytes, platelets, B cells, T cells and monocytes after cell activation, as well as eosinophils, fibroblasts, endothelial cells, keratinocytes and neurons (2). It is a ligand for CD6 and NgCAM but also engages in homophilic interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation (3).

The biological activity of Mouse ALCAM was determined by its ability to block adhesion of HUT-78, a human cutaneous T cell lymphoma, to immobilized rhCD6/Fc protein. rmALCAM/Fc at 10 μg/mL (50 μL/well) blocked 100% of HUT-78 cell adhesion (1 x 106 cells/mL, 50 μL/well) to immobilized rhCD6/Fc (10 μg/mL, 100 μL/well).

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Trp28

Lyophilized

The predicted molecular weight of Recombinant Mouse ALCAM is Mr 82 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 114-122 kDa.

82

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Weichert, W. et al. (2004) J. Clin. Pathol. 57:1160
2. Bowen, MA. et al. (1995) J. Exp. Med. 181:2213
3. Aruffo, A. et al. (1997) Immunol. Today 18:498