Recombinant Mouse B7-H2 (ICOSL)

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Pricing & Details

Product. No.B536
Alternate Names
B7 Homolog 2, B7RP-1, B7h, LICOS, GL50
Expression Host
NS0 cells
Species
Mouse
Prod No.
Size
Price
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B536-100 µg
100 µg
$359.00
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Min: 1
Step: 1

Background

B7-H2, also known as ICOSL and B7RP-1, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains. B7-H2 is expressed on B cells and macrophages. It binds to ICOS on activated T cells and thus delivers a positive costimulatory signal for optimal T cell function (1). ICOS and B7RP-I do not interact with proteins in the CD28-B7 pathway (2). The structural features of B7-H2 are crucial for its costimulatory function. B7-H2 constructs lacking either the IgC or IgV domain demonstrates that receptor binding is mediated solely by the IgV domain but requires the IgC domain for maintaining the structural integrity of the protein (3).

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Fusion Protein Tag
Fc Fusion Protein
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
etev gamvgsnvvl scidphrrhf nlsglyvywq ienpevsvty ylpykspgin vdssyknrgh lsldsmkqgn fslylknvtp qdtqeftcrv fmntatelvk ileevvrlrv aanfstpvis tsdssnpgqe rtytcmskng ypepnlywin ttdnslidta lqnntvylnk lglydvistl rlpwtsrgdv lccvenvalh qnitsisqae sftgnntknp qethnnelkd iegrmdpksc dkthtcppcp apellggpsv flfppkpkdt lmisrtpevt cvvvdvshed pevkfnwyvd gvevhnaktk preeqynsty rvvsvltvlh qdwlngkeyk ckvsnkalpa piektiskak gqprepqvyt lppsrdeltk nqvsltclvk gfypsdiave wesngqpenn ykttppvlds dgsfflyskl tvdksrwqqg nvfscsvmhe alhnhytqks lslspgk
N-terminal Sequence Analysis
Glu47
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse B7-H2 is Mr 53 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 80-90 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Wiendl, H. et al. (2003) Brain 126:1026
2. Yoshinaga, SK. et al. (1999) Nature 402:827
3. Chattopadhyay, KJ. et al. (2006) Immunol. 177:3920
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
B536
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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