Recombinant Mouse Common Gamma Chain

Recombinant Mouse Common Gamma Chain (γc, IL-2Rγ) is essential for research applications involving immune cell development, cytokine signaling, and immunotherapy because it is a shared receptor subunit for multiple interleukins critical to lymphocyte biology.

Key scientific reasons to use recombinant mouse common gamma chain:

• Central role in cytokine signaling: The common gamma chain is a component of receptors for several interleukins (IL-2, IL-4, IL-7, IL-9, IL-15, IL-21), which collectively regulate the proliferation, differentiation, and survival of T cells, B cells, and NK cells. Recombinant protein enables precise study of these pathways in vitro and in vivo.

• Modeling immunodeficiency and immune regulation: Deficiency or mutation in the common gamma chain leads to severe combined immunodeficiency (SCID), highlighting its necessity for lymphocyte development. Recombinant protein can be used to dissect molecular mechanisms underlying immunodeficiencies and to test gene therapy or protein replacement strategies.

• Immunotherapy and cancer research: γc cytokines are being harnessed to enhance anti-tumor immune responses. Recombinant common gamma chain allows for the development and testing of novel immunotherapeutic approaches, such as engineered cytokine fusion proteins or CAR-T cell modifications that depend on γc signaling.

• Functional assays and controls: Recombinant mouse common gamma chain is used as a standard or control in ELISA, Western blot, and cell-based assays to ensure reproducibility and specificity when studying cytokine-receptor interactions or screening for neutralizing antibodies.

• NK cell biology: γc cytokines are crucial for NK cell development and function. Recombinant protein supports research into NK cell-based therapies and the regulation of specialized immune subsets.

Typical applications include:

• Cell signaling studies (e.g., STAT phosphorylation, receptor binding assays)
• Functional assays for lymphocyte proliferation, differentiation, and survival
• Immunodeficiency disease modeling
• Immunotherapy development (e.g., cytokine fusion proteins, CAR-T engineering)
• Standardization and validation of immunoassays (ELISA, Western blot)

Best practices: Use recombinant protein with appropriate carrier (e.g., BSA) for cell culture or as an ELISA standard to maintain stability and bioactivity. Validate specificity and activity in your assay system, as cross-reactivity with human proteins may occur.

In summary, recombinant mouse common gamma chain is a foundational tool for dissecting and manipulating immune cell biology, modeling disease, and developing advanced immunotherapies.

You can use recombinant Mouse Common Gamma Chain as a standard for quantification or calibration in your ELISA assays, provided that the recombinant protein is highly purified, its concentration is accurately determined, and it is compatible with the antibodies and assay format you are using.

Key considerations and supporting details:

• Assay Calibration: Commercial ELISA kits for Mouse Common Gamma Chain/IL-2Rγ are calibrated using highly purified recombinant protein, typically expressed in mammalian cells (e.g., HEK293). This recombinant standard is used to generate the standard curve for quantification, indicating that recombinant protein is suitable for this purpose if it matches the assay’s requirements.

• Purity and Quantification: The recombinant protein used as a standard should be of high purity and its concentration should be accurately measured, ideally by methods such as HPLC or absorbance at 280 nm with a known extinction coefficient. Impurities or inaccurate quantification can lead to errors in your standard curve and sample measurements.

• Antibody Recognition: The ELISA antibodies must recognize both the recombinant and natural forms of the Mouse Common Gamma Chain. Commercial assays validate that their antibodies detect both forms, but if you are developing a custom assay, you must confirm this cross-reactivity.

• Matrix Effects: When using recombinant protein as a standard, ensure that the standard diluent matches the sample matrix as closely as possible to minimize matrix effects and improve quantification accuracy.

• Controls: It is recommended to include endogenous positive controls (e.g., mouse serum or cell lysate with known gamma chain levels) to validate that your standard curve accurately reflects quantification in biological samples.

• Lot-to-Lot Variation: The standard curve range and sensitivity may vary between lots of recombinant protein, so always refer to the lot-specific data and revalidate your standard curve with each new lot.

In summary, recombinant Mouse Common Gamma Chain is appropriate as a standard for ELISA quantification if it is highly purified, accurately quantified, and validated for recognition by your assay antibodies. Always confirm compatibility with your specific assay system and include appropriate controls for reliable quantification.

Recombinant Mouse Common Gamma Chain has been validated for several key applications in published research, primarily in immunology and cell biology. The most frequently reported applications include:

• ELISA (Enzyme-Linked Immunosorbent Assay): Used as a standard or control for quantifying cytokine receptor levels or for detecting interactions with gamma-chain cytokines.
• Western Blot: Utilized as a control protein or for validating antibody specificity against the common gamma chain.
• Cell Culture Assays: Applied in functional studies to assess cytokine signaling, lymphocyte proliferation, and receptor-ligand interactions, often in the context of T cell or NK cell development and function.
• Immunotherapy and Drug Development Models: Used in preclinical models to study the effects of gamma-chain cytokines (such as IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21) on immune cell populations, cancer immunotherapy, and gene therapy for immunodeficiencies.

Supporting Details:

• The common gamma chain (γc, CD132) is a shared receptor subunit for multiple interleukin receptors (IL-2, IL-4, IL-7, IL-9, IL-15, IL-21), making it central to studies of lymphocyte development, immune signaling, and cytokine biology.
• Recombinant forms are often used to dissect signaling pathways, validate antibody specificity, and serve as controls in quantitative assays.
• In cell-based assays, recombinant gamma chain proteins help elucidate mechanisms of cytokine-induced proliferation, differentiation, and survival of T cells, B cells, and NK cells.
• In translational research, recombinant gamma chain and its associated cytokines are being explored for therapeutic modulation of immune responses in cancer, autoimmunity, and immunodeficiency disorders.

Additional Relevant Information:

• Recombinant gamma chain proteins are also used in structural and biochemical studies to map receptor-cytokine interactions and downstream signaling events.
• In gene therapy and knockout mouse models, the gamma chain is critical for understanding severe combined immunodeficiency (SCID) and related pathologies.
• The protein is typically supplied carrier-free for cell culture or with BSA for ELISA and other biochemical assays, depending on experimental requirements.

In summary, ELISA, Western blot, cell culture assays, and immunological functional studies are the principal validated applications for recombinant mouse common gamma chain in published research, with broad utility in both basic and translational immunology.

To reconstitute and prepare Recombinant Mouse Common Gamma Chain protein for cell culture experiments, dissolve the lyophilized protein in sterile buffer—typically sterile PBS or sterile distilled water—to a concentration recommended by the manufacturer, often in the range of 0.1–1.0 mg/mL. For optimal stability and activity, include a carrier protein such as 0.1%–1% BSA if specified.

Step-by-step protocol:

• Centrifuge the vial briefly to collect all lyophilized powder at the bottom before opening.
• Add sterile buffer (e.g., PBS or distilled water) to achieve the desired concentration, commonly 500 μg/mL for gamma chain proteins. If the datasheet specifies, use PBS with at least 0.1% BSA as a carrier to prevent adsorption and loss of activity.
• Gently mix by swirling or tapping (do not vortex) until the powder is fully dissolved.
• Aliquot the solution to avoid repeated freeze-thaw cycles, which can degrade the protein.
• Storage: Store aliquots at –80 °C for long-term use or at 4–8 °C for short-term (2–7 days). Avoid multiple freeze-thaw cycles to preserve activity.

Additional considerations for cell culture:

• Before adding to cell cultures, dilute the reconstituted stock to the working concentration using cell culture medium (e.g., RPMI 1640 or DMEM with serum and supplements as appropriate for your assay).
• If the protein is sensitive or if the datasheet recommends, filter-sterilize the final working solution using a 0.2 μm filter.
• Always consult the specific product datasheet for any unique requirements, such as buffer composition, pH, or additives.

Summary of best practices:

• Use sterile technique throughout to prevent contamination.
• Include carrier protein (BSA or HSA) if recommended to stabilize the protein.
• Aliquot and freeze at –80 °C for long-term storage; avoid repeated freeze-thaw cycles.
• Dilute in cell culture medium immediately before use.

If your product datasheet specifies a different buffer or concentration, always follow those instructions for optimal results. If no datasheet is available, PBS with 0.1% BSA at 0.1–1.0 mg/mL is a generally accepted starting point for recombinant cytokine and receptor proteins in cell culture.