Anti-Mouse Common Gamma Chain

Anti-Mouse Common Gamma Chain

Product No.: C1477

[product_table name="All Top" skus="C1477"]

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Target
Common Gamma Chain
Product Type
Polyclonal Antibody
Alternate Names
Common Cytokine Receptor Gamma Chain, CD132, Interleukin 2 Receptor Gamma Chain (IL-2 Rγ)
Applications
ELISA Indirect
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N
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WB

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Data

Anti-Mouse Common Gamma Chain Bioactivity Data
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
Sf 21-Derived Recombinant Mouse Gammac Extracellular Domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse Common Gamma Chain this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse Common Gamma Chain is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse Common Gamma Chain bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse Common Gamma Chain (at 0.8 ng/ml) is 6 - 24 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse Common Gamma Chain recognizes Mouse Common Gamma Chain. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
The common gamma-chain (gamma(c)) is an indispensable subunit of the functional receptor complexes for IL-4, IL-7, IL-9, and IL-15 as well as IL-2.1 It is a cytokine receptor subunit that is located on the surface of certain sub-sets of lymphocytes (white blood cells). Common gamma-chain is critical for lymphocyte development.

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Sugamura, K. et al. (2001) J Immunol. 167: 1
Indirect Elisa Protocol
N
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.