Recombinant Mouse IFNγ

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Pricing & Details

Product. No.I-217
Alternate Names
Interferon Gamma, Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI
Expression Host
E. coli
Prod No.
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I-217-100 µg
100 µg
In stock
Min: 1
Step: 1
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Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3

Protein Details

>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse Interferon-Gamma was measured in an anti-viral assay using L-929 cells infected with EMC virus.1 The expected ED50 for this effect is 0.3 - 0.9 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
hgtviesles lnnyfnssgi dveekslfld niwrnwqkdg dmkilqsqii sfylrlfevl kdnqaisnni svieshlitt ffsnskakkd afmsiakfev nnpqvqrqaf nelirvvhql lpesslrkrk rsrc
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse IFN-γ is Mr 15.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 11-12 kDa (reducing conditions).
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) with trehalose pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -80°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -80°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Soong, L. et al. (2004) Infect Immun.72(2):988-95. PubMed
2. Goeddel, DV. et al. (1982) Nature 298: 859
3. Wilson, CB. et al. (2007) Adv. Immunol. 96: 41
4. Hume, DA. et al. (2004) J Leukoc Biol. 75: 163
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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