Recombinant Mouse IFNγ

Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.¹ It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.² IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.³

The biological activity of Mouse Interferon-Gamma was measured in an anti-viral assay using L-929 cells infected with EMC virus.¹ The expected ED₅₀ for this effect is 0.3 - 0.9 ng/ml.

hgtviesles lnnyfnssgi dveekslfld niwrnwqkdg dmkilqsqii sfylrlfevl kdnqaisnni svieshlitt ffsnskakkd afmsiakfev nnpqvqrqaf nelirvvhql lpesslrkrk rsrc

Met

Lyophilized

The predicted molecular weight of Recombinant Mouse IFN-γ is Mr 15.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 11-12 kDa (reducing conditions).

15.6

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) with trehalose pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -80°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -80°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Soong, L. et al. (2004) Infect Immun.72(2):988-95. PubMed
2. Goeddel, DV. et al. (1982) Nature 298: 859
3. Wilson, CB. et al. (2007) Adv. Immunol. 96: 41
4. Hume, DA. et al. (2004) J Leukoc Biol. 75: 163