Recombinant Mouse IGFBP-3
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BackgroundInsulin-like growth factor binding protein 3, also known as IGFBP3, is a member of the insulin-like growth factor binding protein (IGFBP) family that are cysteine-rich proteins that act as a carrier protein. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Protein DetailsPurity >95% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.01 EU/µg as determined by the LAL method Biological Activity The biological activity of Mouse IGFBP-3 was determined by its ability to inhibit the biological activity of rmIGF-II on MCF-7 cells (Karey, K.P. et al., 1988, Cancer Research 48:4083). The expected ED<sub>50</sub> for this effect is typically 0.125 - 0.5 μg/ml in the presence of 30 ng/ml rmIGF-II. Protein Accession No. Amino Acid Sequence ppvaragag avgagpvvrc epcdaralsq cappptapac telvrepgcg ccltcalreg dacgvyterc gtglrcqprp aeqyplrall ngrgfcanas aagslstylp sqpapgnise seeehnagsv esqvvpsthr vtdskfhplh akmdvikkgh ardsqrykvd yesqstdtqn fsseskrete ygpcrremed tlnhlkflnv lsprgvhipn cdkkgfykkk qcrpskgrkr gfcwcvdkyg qplpgydtkg kddvhclsvq s N-terminal Sequence Analysis Pro22 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Mouse IGFBP-3 is Mr 29.4 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 40-50 kDa. Predicted Molecular Mass 29.4 Formulation This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA). Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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