Recombinant Human IGFBP-3

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Pricing & Details

Product. No.I-350
Alternate Names
Insulin-Like Growth Factor Binding Protein 3, Growth-Hormone-Dependant Binding Protein, BP-53, IBP3
Expression Host
NS0 cells
Species
Human
Prod No.
Size
Price
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Qty
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I-350-25 µg
25 µg
$349.00
In stock
Max:
Min: 1
Step: 1

Background

Insulin-like growth factor binding protein 3, also known as IGFBP3, is a member of the insulin-like growth factor binding protein (IGFBP) family that are cysteine-rich proteins that act as a carrier protein. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors.

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IGFBP-3 was determined by its ability to inhibit the biological activity of rhIGF-I or rhIGF-II on MCF-7 cells (Karey, K.P. et al., 1988, Cancer Research 48:4083). The expected ED50 for this effect is typically 0.05 - 0.15 μg/ml in the presence of 14 ng/ml rhIGF-II.
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
gas sgglgpvvrc epcdaralaq capppavcae lvrepgcgcc ltcalsegqp cgiytercgs glrcqpspde arplqalldg rglcvnasav srlrayllpa ppapgnases eedrsagsve spsvssthrv sdpkfhplhs kiiiikkgha kdsqrykvdy esqstdtqnf sseskretey gpcrremedt lnhlkflnvl sprgvhipnc dkkgfykkkq crpskgrkrg fcwcvdkygq plpgyttkgk edvhcysmqs k
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IGFBP-3 is Mr 29 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 37-47 kDa.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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