Recombinant Mouse IGFBP-3
Insulin-like growth factor binding protein 3, also known as IGFBP3, is a member of the insulin-like growth factor binding protein (IGFBP) family that are cysteine-rich proteins that act as a carrier protein. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors.
>95% by SDS-PAGE and analyzed by silver stain.
<0.01 EU/µg as determined by the LAL method
The biological activity of Mouse IGFBP-3 was determined by its ability to inhibit the biological activity of rmIGF-II on MCF-7 cells (Karey, K.P. et al., 1988, Cancer Research 48:4083). The expected ED50 for this effect is typically 0.125 - 0.5 μg/ml in the presence of 30 ng/ml rmIGF-II.
Protein Accession No.
Amino Acid Sequence
ppvaragag avgagpvvrc epcdaralsq cappptapac telvrepgcg ccltcalreg dacgvyterc gtglrcqprp aeqyplrall ngrgfcanas aagslstylp sqpapgnise seeehnagsv esqvvpsthr vtdskfhplh akmdvikkgh ardsqrykvd yesqstdtqn fsseskrete ygpcrremed tlnhlkflnv lsprgvhipn cdkkgfykkk qcrpskgrkr gfcwcvdkyg qplpgydtkg kddvhclsvq s
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse IGFBP-3 is Mr 29.4 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 40-50 kDa.
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.