Recombinant Mouse IL-12

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Pricing & Details

Product. No.I-1180
Alternate Names
NKSF, CLMF
Expression Host
HEK 293 cells
Species
Mouse
Prod No.
Size
Price
Avail.
Qty
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I-1180-10 µg
10 µg
$224.00
In stock
Max:
Min: 1
Step: 1
I-1180-100 µg
100 µg
$1,610.00
In stock
Max:
Min: 1
Step: 1
I-1180-500 µg
500 µg
$4,500.00
In stock
Max:
Min: 1
Step: 1
I-1180-1.0 mg
1.0 mg
$8,550.00
In stock
Max:
Min: 1
Step: 1

Background

IL-12 plays a role in resistance against pathogens via the differentiation of naive T cells into Th1 cells. It stimulates the growth and function of T cells, blocks formation of new blood vessels, and contributes to antimycobacterial immune response. In addition, it promotes the production of IFN-γ and TNF-α and reduces IL-4 mediated suppression of IFN-γ. Consequently, this enhances the immunostimulatory and immunomodulatory effects of IFN-γ. In addition, there appears to be a link between IL-2 and the signal transduction of IL-12 in NK cells, which enhances the functional response of IL-12 via IFN-γ production and killing of target cells. Furthermore, IL-12 is thought to be associated with autoimmunity. IL-12 was shown to worsen the condition when administered to people already suffering from autoimmune diseases. Comparatively, inhibition of IL-12 (either through IL-12 gene knock-out in mice or treatment of mice with IL-12 mAbs) improved the disease.

Protein Details

Purity
>95% by SDS-PAGE and HPLC
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of this rMouse IL-12 proteins was determined by by the induced expression of CD25 on CD8+ mouse T cell . The expected ED50= , corresponding to a specific activity of The activity is determined by the induced expression of CD25 on CD8+ mouse T cells and is typically detectable starting at 0.1 ng/mL
Expression Host
HEK 293 cells
Protein Accession No.
P43432/P43431
Amino Acid Sequence
p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse IL-12 is Mr 70 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.5 with no calcium, magnesium, or preservatives present.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Kapsenberg, ML. et al. (1997) J. Immunol. 159: 28
2. Ritz, J. et al. (2001) Blood. 97(12):3860-6.
3. Barnes, PF. et al. (1994) J Clin Invest. 93(4):1733-9.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.