Recombinant Mouse IL-12 p40 (Homodimer)

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Pricing & Details

Product. No.I-396
Alternate Names
Interleukin-12 p40 (Homodimer), NKSF, TSF, Maturation Factor, Cytotoxic Lymphocyte Maturation Factor (CLMF)
Expression Host
Sf 21 Insect Cells
Species
Mouse
Prod No.
Size
Price
Avail.
Qty
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I-396-25 µg
25 µg
$389.00
In stock
Max:
Min: 1
Step: 1

Background

Interleukin 12 (IL-12, NK cell stimulatory factor, cytotoxic lymphocyte maturation factor) is a heterodimeric cytokine that is naturally produced by dendritic cells1 , macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th1 cells, which is important in resistance against pathogens. It is known as a T cell stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 also has anti-angiogenic activity, which can block the formation of new blood vessels. IL-12 binds to the IL-12 receptor and upon binding, IL-12R-β2 becomes tyrosine phosphorylated and provides binding sites for kinases, Tyk2 and Jak2. These are important in activating critical transcription factor proteins such as STAT4 which are implicated in IL-12 signaling in T cells and NK cells.2 IL-12 contributes to the antimycobacterial immune response by enhancing production of interferon-gamma, facilitating development of Th1 cells and augmenting cytotoxicity of antigen-specific T cells and natural killer cells.3

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse IL-12 p40 was determined by its ability to inhibit the IL-12 dependent proliferation of mouse splenocytes activated with PHA and IL-2. The expected ED50 is typically 1-3 ng/ml in the presence of 0.3 ng/ml of mouse IL-12.
Expression Host
Sf 21 Insect Cells
Protein Accession No.
Amino Acid Sequence
mwelekdv yvvevdwtpd apgetvnltc dtpeedditw tsdqrhgvig sgktltitvk efldagqytc hkggetlshs hlllhkkeng iwsteilknf knktflkcea pnysgrftcs wlvqrnmdlk fniksssssp dsravtcgma slsaekvtld qrdyekysvs cqedvtcpta eetlpielal earqqnkyen ystsffirdi ikpdppknlq mkplknsqve vsweypdsws tphsyfslkf fvriqrkkek mketeegcnq kgaflvekts tevqckggnv cvqaqdryyn sscskwacvp crvrs
N-terminal Sequence Analysis
Met23
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse IL-12 p40 is Mr 35.8 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Kapsenberg, ML. et al. (1997) J. Immunol. 159: 28
2. Ritz, J. et al. (2001) Blood. 97(12):3860-6.
3. Barnes, PF. et al. (1994) J Clin Invest. 93(4):1733-9.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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