Recombinant Mouse IL-18 BPd

Recombinant Mouse IL-18 BPd

Product No.: I-547

[product_table name="All Top" skus="I-547"]

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Alternate Names
Interleukin 18 Binding Protein d, MC54L, Igifbp, IL-18BP, Il18bp
Product Type
Recombinant Protein
Expression Host
NS0 Cells
Species
Mouse

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Select Product Size
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Background

IL-18-binding protein (IL-18BP) is a secreted glycoprotein in the IL-1 family of ligands, however, it bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. IL-18BP functions as a natural anti-inflammatory and immunosuppressive molecule neutralizing the effects of high IL-18 levels during inflammation (1). It is constitutively expressed and secreted in mononuclear cells and can be enhanced by IFN gamma, which suggests IL-18 activity is modulated by a negative feedback mechanism via IL-18BP. There are four known variants of IL-18BP (a, b, c & d). The isoform IL-18BPd, also known as MC54L, lacks a complete Ig domain and the ability to bind or neutralize IL-18 (2). IL-18BPd has been shown to be produced as a secreted full-length molecule that binds cell surface glycosaminoglycans in the extracellular matrix and also as a furin-cleaved form that contains only the IL-18 binding domain (3). It is related to a secreted viral protein, vIL-18BP, and may contribute to the lack of inflammatory responses characteristic of molluscum contagiosum virus lesions (4).

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse IL-18BPd was determined by its ability to inhibit the IL-18 induced response of mouse T cells. The expected ED<sub>50</sub>= 0.1-0.4 ug.
Fusion Protein Tag
Fc Fusion Protein
Protein Accession No.
Amino Acid Sequence
tsap qttatvltgs skdpcsswsp avptkqypal dviwpekevp lngtltlsct acsrfpyfsi lywlgngsfi ehlpgrlkeg htsrehrnts twlhralvle elsptlrstn fsclfvdpgq vaqyhiilaq lwdglktapp psqetlsshs pvsrsagpgv aiegrmdpks cdkthtcppc papellggps vflfppkpkd tlmisrtpev tcvvvdvshe dpevkfnwyv dgvevhnakt kpreeqynst yrvvsvltvl hqdwlngkey kckvsnkalp apiektiska kgqprepqvy tlppsrdelt knqvsltclv kgfypsdiav ewesngqpen nykttppvld sdgsfflysk ltvdksrwqq gnvfscsvmh ealhnhytqk slslspgk
N-terminal Sequence Analysis
Thr27 & Ala29
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse IL-18 BPd is Mr 44.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 60-70 kDa.
Predicted Molecular Mass
44.5
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Novick, D. et al. (1999) Immunity 10:127
2. Kim, SH. et al. (2000) Proc. Nat. Acad. Sci. (USA) 197:1190
3. Xiang, Y. et al. (2003) J. Virol. 77:2623
4. Xiang, Y. et al. (1999) Proc. Nat. Acad. Sci. (USA) 96:11537

Certificate of Analysis

IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Prod No.
Description
I-547
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.