Recombinant Rat IL-17

Recombinant Rat IL-17 (particularly IL-17A and IL-17F) is a valuable tool for research applications due to its central role in inflammation, immune regulation, and disease pathogenesis. Here are key reasons why you should consider using Recombinant Rat IL-17 in your studies:

1. Modeling Inflammatory and Immune Responses

• IL-17 is a proinflammatory cytokine that drives the recruitment of immune cells (especially neutrophils) and the production of chemokines, cytokines, and antimicrobial peptides.
• Using recombinant IL-17 allows you to directly investigate the effects of this cytokine in vitro and in vivo, mimicking inflammatory conditions such as autoimmune diseases, infections, and tissue injury.

2. Studying Disease Mechanisms

• IL-17 plays a critical role in diseases like rheumatoid arthritis, psoriasis, colitis, and neuroinflammatory disorders.
• By applying recombinant IL-17, you can dissect the molecular pathways involved (e.g., NF-κB activation, chemokine production) and evaluate the contribution of IL-17 to disease progression or protection.

3. Testing Therapeutic Interventions

• Recombinant IL-17 is essential for preclinical studies evaluating the efficacy of anti-IL-17 therapies (e.g., antibodies, receptor blockers, small molecule inhibitors).
• It enables you to assess how blocking IL-17 signaling affects inflammation, immune cell infiltration, and tissue damage.

4. Investigating Tissue-Specific Effects

• IL-17 acts on a variety of cell types, including epithelial cells, fibroblasts, and neurons.
• You can use recombinant IL-17 to study its effects on barrier function, tissue repair, and neuroinflammation in different organ systems.

5. Standardization and Reproducibility

• Recombinant proteins provide a consistent and well-characterized source of IL-17, ensuring reproducible experimental conditions across studies and laboratories.

6. Species-Specific Research

• Recombinant Rat IL-17 is specifically designed for use in rat models, making it ideal for translational research where rat models are preferred over mice or other species.

7. Exploring IL-17 Family Members

• Recombinant IL-17A, IL-17F, and IL-17A/F heterodimers are available, allowing you to compare the biological activities of different IL-17 family members and their roles in health and disease.

8. Supporting Drug Discovery and Development

• Recombinant IL-17 is used in high-throughput screening assays to identify novel compounds that modulate IL-17 signaling, supporting the development of new therapeutics for inflammatory and autoimmune diseases.

In summary, Recombinant Rat IL-17 is a powerful reagent for studying inflammation, immune regulation, and disease mechanisms, and it supports the development and evaluation of targeted therapies in preclinical research.

Yes, recombinant rat IL-17A can be used as a standard for quantification or calibration in ELISA assays, provided it is validated for this purpose and matches the assay requirements.

Supporting details:

• ELISA kits for rat IL-17A are specifically designed to quantify both natural and recombinant rat IL-17A in biological samples, and their protocols routinely use recombinant rat IL-17A as the standard for generating calibration curves.
• Recombinant rat IL-17A proteins are often supplied in formulations suitable for use as ELISA standards, typically lyophilized and reconstituted in buffer containing carrier proteins such as BSA to ensure stability and reproducibility.
• The standard curve in ELISA is generated by serially diluting the recombinant protein standard and measuring its signal, which is then used to interpolate the concentration of IL-17A in unknown samples.
• It is important to ensure that the recombinant standard is compatible with the antibodies used in your ELISA kit. Most commercial kits specify that their antibodies recognize both natural and recombinant forms of rat IL-17A.
• Biological activity and purity: Recombinant rat IL-17A used as a standard should be of high purity and, ideally, validated for biological activity to ensure accurate quantification.

Best practices:

• Always follow the ELISA kit manufacturer's recommendations regarding the preparation and use of standards.
• Prepare the standard curve with the recombinant rat IL-17A in the same matrix as your samples (e.g., serum, plasma, or buffer) to minimize matrix effects.
• Run standards in duplicate or triplicate for accuracy and reproducibility.
• If using a recombinant standard from a different source than the kit, confirm that it is recognized equivalently by the kit's antibodies and that its concentration is accurately known.

In summary: Recombinant rat IL-17A is widely used and accepted as a standard for ELISA quantification, provided it is properly validated and prepared according to assay requirements.

Recombinant Rat IL-17 (typically IL-17A or IL-17A/F) has been validated for several key applications in published research, including ELISA, functional (bioactivity) assays, in vivo studies, and immunoassays.

Validated Applications:

• ELISA (Enzyme-Linked Immunosorbent Assay):

  • Used as a standard or control for quantifying IL-17A in rat serum or tissue samples.
  • Recovery and linearity studies confirm its suitability for accurate cytokine measurement in biological samples.

• Functional/Bioactivity Assays:

  • Demonstrated to induce IL-6 production in NIH-3T3 cells, confirming biological activity.
  • Used to study downstream signaling pathways such as NF-κB and MAP kinase activation.
  • Applied in chemokine induction and neutrophil recruitment assays, reflecting its role in inflammation and host defense.

• In Vivo Studies:

  • Administered to rats to model or modulate inflammatory and autoimmune diseases, such as experimental arthritis, colitis, and neuroinflammation.
  • Used to investigate effects on hypertension, vascular dysfunction, and immune cell activation in animal models.
  • Applied in studies of cognitive function and neuroinflammation in anesthetized rats.

• Immunohistochemistry and Western Blot:

  • Validated for detection and quantification of IL-17A in tissue sections and protein extracts.

Additional Context:

• Mechanistic Studies: Recombinant rat IL-17A is widely used to dissect its role in immune signaling, including induction of pro-inflammatory cytokines (e.g., IL-6, G-CSF), chemokines, and antimicrobial peptides.
• Disease Models: Frequently employed in models of rheumatoid arthritis, multiple sclerosis, asthma, and otitis media to study pathogenesis and therapeutic interventions.
• Cellular Assays: Used to stimulate various cell types (e.g., fibroblasts, epithelial cells) to assess cytokine and chemokine production, barrier function, and immune cell recruitment.

Summary Table:

If you need details on a specific disease model or assay protocol, please specify the context.

To reconstitute and prepare Recombinant Rat IL-17 protein for cell culture experiments, dissolve the lyophilized protein in sterile water or PBS at a concentration not exceeding 1 mg/mL, then further dilute to your desired working concentration using cell culture-compatible buffers.

Essential protocol details:

• Reconstitution solvent:
  • For most recombinant rat IL-17A or IL-17A/F proteins, use sterile water or sterile PBS. Some protocols recommend adding at least 0.1% carrier protein (such as bovine serum albumin, BSA) to PBS to stabilize the protein, especially for low concentrations.
• Concentration:
  • Initial reconstitution is typically at 0.1–1 mg/mL. For some preparations, 100–200 μg/mL in PBS is recommended.
• Mixing:
  • Gently pipette the solution down the sides of the vial to dissolve. Do not vortex; allow the protein to dissolve for 15–30 minutes at room temperature with gentle agitation.
• Dilution:
  • After complete dissolution, dilute the stock solution to your desired working concentration using cell culture medium or buffer. For bioactivity assays, typical working concentrations range from 3–23 ng/mL depending on the cell type and experimental design.
• Storage:
  • After reconstitution, aliquot and store at 2–8°C for up to 1 month or -20°C to -80°C for up to 3 months. Avoid repeated freeze-thaw cycles.
• Sterility:
  • Ensure all solutions and containers are sterile to prevent contamination in cell culture.

Best practices:

• If your protein is carrier-free, consider adding carrier protein (e.g., 0.1% BSA) for long-term stability, especially at low concentrations.
• Always centrifuge the vial briefly before opening to collect the lyophilized powder at the bottom.
• Prepare aliquots to avoid repeated freeze-thaw cycles, which can reduce protein activity.
• Confirm the final buffer composition is compatible with your cell culture system (e.g., avoid high salt or non-physiological pH).

Example protocol:

1. Briefly centrifuge the vial before opening.2. Add sterile water or PBS (with 0.1% BSA if needed) to achieve 0.1–1 mg/mL.3. Gently mix by pipetting; do not vortex.4. Allow to dissolve at room temperature for 15–30 minutes.5. Dilute to working concentration in cell culture medium or buffer.6. Aliquot and store unused stock at -20°C or -80°C.

Note: Always consult the specific product datasheet for any unique requirements, as formulation and recommended reconstitution may vary by manufacturer and protein variant.