RIPK2 Inhibitor 1
Data
NFκB gene reporter assay determination of inhibition of MDP stimulated NFκB activity with
#19510-BID002. An approximate EC50 determination for inhibition of MDP driven-NFκB activation is shown.
: Inhibition was carried out for 24-36 hours on Hodgkin’s lymphoma cell line KMH2 revealing
constitutively active RIPK2. This was followed by lysis and immunoprecipitation (IP) with rabbit anti-RIPK2
antibody (#19510) overnight for total RIPK2 pull down. Following Protein G pull down and wash, 32P-γ-ATP
is added and kinase reaction allowed to proceed for 30 minutes at 30°C. Following kinase reaction, SDSPAGE was used to separate out the proteins, gel was then dried and exposed to film. The p60 RIPK2 band
was quantitated.
DSS-induced inflammation can be inhibited by RIPK2 inhibitor 1 at 1ug/g body weight injected
intraperitoneally (IP) at day 3, 5 and 7 (during period of active disease and time of recovery). The Kaplan-Meir survival curve is plotted for Rassf 1a-/- and 1a+/- mice. Both potentially have RIPK2 unchecked in
colonic tissue (and hence elevated inflammation) if Rassf1a is missing. N = 12-20 animals per condition;
p value > 0.001. - -
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BackgroundRIPK2 (Receptor Interacting Protein Kinase 2) is the obligate kinase of the NOD2 intracellular
pathogen recognition pathway receptor and an upstream kinase activator for NFκB via activation
of TAK1. Inhibiting the activity of RIPK2 may be important in controlling inflammatory responses. Protein DetailsState of Matter Powder Predicted Molecular Mass 410.47 g/mol Product Preparation Stocks should be made up in 100% DMSO to 10-20mM. Stocks can be diluted in >12% DMSO to desired concentration or dissolved in 100% olive oil. Storage and Stability Store at -80°C in appropriate aliquots to avoid multiple freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C References & Citations1. Salla M et al. J Pharmacol Exp Ther. 2018. 365:354-367. 2. Larochelle J, et al. J Neuroinflammation. 2023;20(1):221. 3. Salla, M., et al. Int. J. Mol. Sci. 2023; 24, 5967. 4. Seo B.A., et al. bioRxiv 2024:02.19.580982. 5. Xu Y, et al. Mol Neurobiol. 2024;61(9):6771-6787. 6. You J, et al. Front Pharmacol. 2023;14:1192970. Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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