Anti-Rat EphA5 – Biotin
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Goat Immunogen Purified Recombinant Rat EphA5 (Accession # P54757) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830061 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat EphA5 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Rat EphA5 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E209. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Rat EphA5 recognizes Rat EphA5. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background EPH receptor A5 (EPHA5) is a glycoprotein that is expressed by astrocytes and skeletal muscle. It provides guidance cues for retinal ganglion cells1 and is essential for proper retinal axon guidance and topographic mapping in the mammalian visual system.2 Ephrin A5 serves as a general inhibitor of axonal growth from limbic neurons.3 It also plays a role in motor axon outgrowth and pathfinding to the hindlimb.4,5 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Davenport, RW. et al. (1998) J Neurosci. 18: 975 2. Frisen, J. et al. (1998) Neuron 20: 235 3. Gao, PP. et al. (1998) Proceedings of the National Acad of Sci. 95: 5329 4. Eberhart, J. et al. (2000) Developmental Neurosci. 22: 237 5. Eberhart, J. et al. (2004) J of Neurosci. 24: 1070 Technical Protocols Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
