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Anti-Human CD2 PerCP

Monoclonal Antibody


Prod. No.:C1839
Clone:G11
Isotype:Mouse IgG2a
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Starting at:$85.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CD2 (Clone G11) recognizes an epitope on Human CD2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The CD2 antigen is present on approximately 80% of normal peripheral blood lymphocytes, >95% of thymocytes, 100% of mature circulating T-cells and a subset of NK cells.

Background:
Appropriate pairs of anti-CD2 antibodies will stimulate peripheral T-cell proliferation and effector function.1 The CD2 molecule has been shown to be the receptor for leukocyte function antigen (LFA-3/CD58),2 the binding of which augments T-cell activation mediated by the T-cell antigen receptor (TCR).3 Anti-Human CD2 can be used to deplete CD2 positive cells by complement-mediated cytotoxicity. Analyses of NK lymphocyte subsets and identification of lymphomas and leukemias of T-cell origin have been done using Anti-CD2 antibodies.

Reporter Molecule:
PerCp is a component of the photosynthetic apparatus found in the dinoflagellate Glenodinium. PerCP is a protein complex with a molecular weight of ~35 kDa. Due to its photobleaching characteristics do not use on flow cytometers with high-power lasers.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human CD2 (>98%)
Formulation
This PerCP conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This PerCP conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Meuer, S.C. et al. (1984) Cell 36:897
2. Peterson, A. and Sneed, B. (1987) Nature 329:842
3. Bierer, B.E. et al.(1989) Annu. Rev Immunol. 7:579
4. Krensky, C.F. et al. (1982) Proc. Natl. Acad. Sci. USA 79:7489
5. Krensky, C.F. et al. (1983) J. Immunol. 131:611
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