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Anti-Mouse CD8 Purified Low Endotoxin Functional Formulation

(Clone YTS-169.4)
Monoclonal Antibody

Prod. No.:C2850
Isotype:Rat IgG2b
Conc.:1.0 - 5.0 mg/ml
Pkg. Size:0.5 mg, 1.0 mg, 5.0 mg, 25 mg, 50 mg, 100 mg
Starting at:$125.00
Availability:In Stock
Storage:2-8°C Detailed storage instructions below.
Rat Anti-Mouse CD8 (Clone YTS-169) recognizes an epitope on Mouse CD8. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The antibody was also test by PCR and IMPACT1 certified (For more information contact your Sales Rep).

Antigen Distribution:
Present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells.1 CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.

Host Species
CBA mouse thymocytes
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
>95% by SDS-PAGE and HPLC
<0.5 EU/mg as determined by the LAL method
Storage and Stability
This antibody is stable for at least four (4) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), In vivo Depletion
Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections but the concentration is assay dependent.
Each investigator should determine their own optimal working dilution for specific applications.

Country of Origin
1. Nakayama, K. et al. (1994) Science 263:1131
2. Ledbetter, J. A. et al. (1979) Immunol. Rev. 47:63
3. Ledbetter, J. A. et al. (1980) J. Exp. Med. 152:280
4. Van Ewijk, et al. (1981) J. Immunol. 127:2594
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