PD-1 Introduction
Investigate the anti-tumor effects of PD-1 antibodies in your mouse cancer model research. Our monoclonal Anti-PD-1 antibodies effectively target the Programmed Cell Death Protein 1 (PD-1) receptor, which normally suppresses T-cell activity on tumor cells. Blocking PD-1 reinvigorates anti-tumor immunity, a key mechanism in immunotherapy development.
Anti-mouse PD-1 Antibody Clones
Leinco Technologies offers three (3) unique anti-PD-1 monoclonal antibodies: 29F.1A12 (in vivo GOLD Version), RMP1-14, 384-35 (fully murinized). All three of our PD-1 antibodies are perfectly suited for in vivo blocking of PD-1 signaling in mouse models. They also boast extensive, multi-year publication records that consistently support this application. Clone 384-35 is part of a new class of mouse anti-mouse antibodies that significantly increases survival past 100 days compared to RMP1-14 and shows tumor volume more than 2.5 fold smaller compared to RMP1-14. The available clones all have extensive publication records to support them. Leinco’s anti-mouse PD-1 antibodies are available in both in vivo GOLD™ and in vivo PLATINUM™ formats. When selecting a PD-1 antibody for your mouse in vivo studies, you have excellent options, each with distinct advantages.
Clone RMP1-14: The Gold Standard for In Vivo Blocking
The RMP1-14 clone stands out with the most extensive publication record specifically for in vivo blocking of PD-1 signaling. If your research solely focuses on in vivo PD-1/PD-L pathway inhibition, RMP1-14 is a highly validated and reliable choice.
Clone 29F.1A12: Versatile PD-1 Antibodies for Comprehensive Research
For researchers needing broader applications, our 29F.1A12 clone offer remarkable versatility. Both have strong publication records for in vivo PD-1 blocking, similar to RMP1-14. However, their utility extends far beyond. This PD-1 antibody is also suitable for:
- In vitro PD-1 neutralization
- Western blotting
- Immunohistochemistry
- Immunofluorescence
- Flow cytometry
This multi-application capability is a significant advantage if you plan to run in vitro or diagnostic experiments alongside your in vivo studies, streamlining your workflow and maximizing your research potential.
Which PD-1 Clone Is Best For My Research?
At Leinco Technologies, all of our PD-1 antibodies are meticulously developed and quality tested to bind to PD-1 and meet the lower endotoxin levels and highest purity levels for mouse in vivo experiments. However, depending on your specific mouse model and research requirements, one PD-1 antibody clone may be better suited for your needs.
While each of the PD-1 antibodies available in our catalog were developed by different researchers at various times, they were all consistently produced using established hybridoma technology, ensuring reliable and high-quality reagents for your studies.
| Clone | RMP1-14 | RMP1-30 | 384-35 | 29F.1A12 |
|---|---|---|---|---|
| in vivo Products | PLATINUM™ - P372 GOLD™ - P362 | GOLD™ - C3442 | Recombinant in vivo - P710 | PLATINUM™ - P378 GOLD™ - P377 |
| Leinco Catalog | RMP1-14 Products | RMP1-30 Products | 384-35 Products | 29F.1A12 Products |
| Isotype | Rat IgG2a, k | Rat IgG2b κ | Mouse IgG1 | Rat IgG2a |
| Original Citation | Was first published in 2005 by Takanori Kanai and Hideo Yagit, et al. | Leinco Co-developed | Was first published in 2003 by Spencer C. Liang and Gordon Freeman et al. | |
| Immunogen | Syrian hamster BKH cells transfected with mouse PD-1 cDNA. | Syrian hamster BKH cells transfected with mouse PD-1 cDNA. | Recombinant mouse PD-1 used in PD-1 -/- knockout mouse | Recombinant mouse PD-1-Ig fusion protein. |
| Purity | >95% Purity by SDS Page and HPLC | >95% Purity by SDS Page and HPLC | >95% Purity by SDS Page and HPLC | >95% Purity by SDS Page and HPLC |
| Preparation | Contains no preservatives or carrier proteins | Contains no preservatives or carrier proteins | Contains no preservatives or carrier proteins | Contains no preservatives or carrier proteins |
| Recommended Isotype Control | Rat IgG2a PLATINUM Or Rat IgG2a GOLD | Rat IgG2b GOLD | Mouse IgG1 GOLD | Rat IgG2a PLATINUM Or Rat IgG2a GOLD |
| Recommended Dilution Buffer | D230 | D230 | D230 | D230 |
| Applications | Clone RMP1-14 is a good Fluorescence-Activated Cell Sorting (FACS) mab and a good blocker of PD-L1 or PD-L2 binding to PD-1; Effective in immunotherapy experiments. NOTE: We would not suggest using this clone to stain cells from a 29F.1A12-treated animal or vice versa. | Clone 384-35 has been used for in vivo functional assay for blocking the binding of PD-L1 to PD-1. | 29F.1A12, PD-1 mAb (rat IgG2a isotype), is a good FACS mab and a good blocker of PD-L1 or PD-L2 binding to PD-1; Effective in immunotherapy experiments. This clone can also work very well in western blots. |
