7-AAD Viability Staining Solution

Pricing & Details

Product No.A431
Formats AvailableView All
Product Type
Cytometry Workflow Solutions
Applications
FC
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A431-200 tests
200 tests
$25.00
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Min: 1
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A431-500 tests
500 tests
$50.00
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Min: 1
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Product Details

Storage and Handling
Store this solution between 2°C and 8°C and protect from light.

Description

Background
7-AAD has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter). 7-AAD Viability Staining Solution can be used as a viability probe for methods of nonviable cell exclusion.
Directions for Use
For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 µl of 7-AAD per million cells and incubate for 5-10 minutes in the dark before analysis.
Hazard Description
CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.

Related Protocols

FC
Products are for research use only. Not for use in diagnostic or therapeutic procedures.