Anti-Aflatoxin B1 [AFA-1]

Purified aflatoxin B1.

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Liquid

Purified by Protein G affinity chromatography

This antibody is stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Next Day 2-8°C

The Clone AFA-1 is a monoclonal antibody specific to aflatoxin B1. Cross-reactivity with other aflatoxins is no known.

Aflatoxins are naturally occurring mycotoxins, primarily produced by Aspergillus flavus and Aspergillus parasiticus molds. These potent toxins commonly contaminate agricultural crops like peanuts, maize, and tree nuts. The aflatoxin molecule possesses an approximate molecular mass of 312.27 g/mol. High moisture and warm temperatures promote mold growth, escalating contamination risk. Laboratories utilize techniques such as ELISA and specialized aflatoxin test kits for their identification and quantification in various samples.

Aflatoxin Toxicity: Impact on Cellular Health and Liver Function Aflatoxins are potent mycotoxins with significant deleterious effects on living organisms. These compounds induce oxidative stress through the generation of reactive oxygen species (ROS), thereby impairing crucial cellular functions. A key mechanism of their toxicity involves direct interaction with the cellular machinery; aflatoxins form DNA adducts that profoundly disrupt both transcription and replication. While aflatoxins do not form traditional biological complexes, their engagement with cellular components considerably modifies the intracellular environment. This interaction and subsequent cellular damage occur predominantly in liver cells, where the process of bioactivation yields highly toxic intermediates, further contributing to hepatotoxicity.