This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Anthrax Protective Antigen (Anthrax PA) recognizes an epitope in the intermediate domain of Anthrax PA. This polyclonal antibody was purified using affinity chromatography.
Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF)1,2. PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8)/anthrax toxin receptor (ATR)3 and capillary morphogenesis protein 2 (CMG2),4 although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin-like protease. The bound fragment binds both LF and EF; the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm. These toxins are usually sufficient to cause rapid cell death, and often the death of the organism.
References & Citations
1. Schwartz, M. N. (2001) New Engl. J. Med. 345:1621 2. Moayeri, M. et al. (2004) Curr. Opin. Microbiol. 7:19 3. Bradley, K. A. et al. (2001) Nature 414:225 4. Scobie, H. M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5170
Products are for research use only. Not for use in diagnostic or therapeutic procedures.