Anti-ATF2 (Phospho-Thr69 or 51) Antibody (43030)

Peptide sequence that includes phosphorylation sites of threonine 69 or 51(D-Q-T(p)- P-T) derived from human ATF2 and conjugated to KLH.

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Liquid

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

This antibody is stable for at least one (1) year at -20°C.

Next Day 2-8°C

Rabbit Polyclonal Antibody specific to ATF2 (Phospho-Thr69 or 51)

ATF2 is a transcription factor that is a member of the leucine zipper family of DNA binding protein that can perform multiple functions. It can bind to the cAMP-responsive element (CRE), and it can form a homodimer or a heterodimer with c-Jun and stimulate CRE-dependent transcription. ATF2 is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro. ATF2 may also be involved in a cell's DNA damage response independent of its role in transcriptional regulation.

Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed:10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type. {PubMed:10821277, PubMed:15916964, PubMed:18397884, PubMed:22304920}.