Anti-ATM Antibody (13014)
Data
Detection of human ATM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-ATM antibody 13014 (lot 13014-2) used for IP at 3 µg per reaction. ATM was also immunoprecipitated by goat anti-ATM antibody 13012. For blotting immunoprecipitated ATM, 13014 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human ATM by Western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-ATM antibody 13014 (lot 13014-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. - -
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse Host Species Goat Immunogen Synthetic peptide representing a portion of the protein encoded within exon 53 (LocusLink ID 472). Product Concentration Lot Specific Formulation Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide State of Matter Liquid Product Preparation Purified by immunoaffinity chromatography Storage and Handling Store at 2 - 8°C. Antibody is stable at 2 - 8°C for 1 year. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Western Blot: 0.1 - 0.4 ug/ml.
Immunoprecipitation: 10 - 20 ug/mg lysate Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat polyclonal antibody specific to ATM of human and mouse. Antigen DetailsFunction Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. RPubMed:10550055, PubMed:10766245, PubMed:10802669, PubMed:10839545, PubMed:10910365, PubMed:10973490, PubMed:11375976, PubMed:12086603, PubMed:12556884, PubMed:14871926, PubMed:15448695, PubMed:15456891, PubMed:15916964, PubMed:16086026, PubMed:16858402, PubMed:17923702, PubMed:19431188, PubMed:19965871, PubMed:26774286, PubMed:29203878, PubMed:30612738, PubMed:30886146, PubMed:9733514, PubMed:9733515, PubMed:9843217}. NCBI Gene Bank ID UniProt.org Research Area Enzymes References & CitationsTechnical Protocols  |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
