Anti-CD73/5’Nucleotidase [10f1]
Anti-CD73/5’Nucleotidase [10f1]
Product No.: 90006
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Clone 10f1 Target CD73/5'Nucleotidase Formats AvailableView All Product Type Monoclonal Alternate Names 5'-NT, EC 3.1.3.5, Ecto-5'-nucleotidase, CD antigen CD73 Isotype IgG1 Applications ELISA , IHC , WB |
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Antibody DetailsProduct DetailsReactivity Species Human Host Species Mouse Immunogen Recombinant 5'-nucleotidase (5'-NT). Accession no. P21589. Formulation Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose, 1mg/ml. State of Matter Lyophilized Product Preparation Purified by Protein A affinity chromatography Storage and Handling This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco ELISA: use at 1-10ug/ml
Immunoblotting: User should use at 1-5ug/ml. A band of ~63kDa is detected. Immunohistochemistry: use at 1-5ug/ml. These are recommended concentrations. End user should determine optimal concentrations for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Monoclonal Antibody specific to CD73/5'-Nucleotidase Background 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. CD73 converts AMP to adenosine. This enzyme consists of a dimer of 2 identical 70-kDa subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. A deficiency of NT5 occurs in a variety of immunodeficiency diseases Antigen DetailsFunction Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. {PubMed:21933152}. NCBI Gene Bank ID UniProt.org Research Area Enzymes References & CitationsTechnical Protocols   |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
