Anti-GABABR1 Antibody (56310)

Product No.: 56310

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Target
GABA(B)R1
Product Type
Polyclonal
Alternate Names
GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1
Isotype
Rabbit
Applications
WB

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Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
Peptide corresponding to aa 33-54 of the GABABR1 subunit.
Product Concentration
Lot Specific
Formulation
PBS, pH 7.4 with 0.02% sodium azide
State of Matter
Liquid
Product Preparation
Purified by Protein A affinity chromatography
Storage and Handling
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at 1:500-1:1,000 dilution. In immunoblots, a band of approximately 120 kD is detected.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to GABA(B)R1
Background
The GABAB receptor is the first discovered G-protein-coupled receptor whose function requires two subunits, R1 and R2, to form a functional receptor. The R1 subunit extracellular domain binds GABA, and the R2 subunit functions in G-protein activation.

Antigen Details

Function
Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:9872316, PubMed:9872744, PubMed:15617512, PubMed:18165688, PubMed:22660477, PubMed:24305054). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:18165688). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10906333, PubMed:10773016, PubMed:10075644, PubMed:9872744, PubMed:24305054). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644). Calcium is required for high affinity binding to GABA (By similarity). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed:9844003). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:9844003, PubMed:9872316, PubMed:10075644, PubMed:9872744, PubMed:22660477). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable). Activated by (-)-baclofen, cgp27492 and blocked by phaclofen (PubMed:9844003, PubMed:9872316, PubMed:24305054). {UniProtKB:Q9Z0U4, PubMed:10075644, PubMed:10773016, PubMed:10906333, PubMed:15617512, PubMed:18165688, PubMed:22660477, PubMed:24305054, PubMed:9844003, PubMed:9872316, PubMed:9872744, ECO:0000305}.; Isoform 1E may regulate the formation of functional GABBR1/GABBR2 heterodimers by competing for GABBR2 binding. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
NCBI Gene Bank ID
UniProt.org
Research Area
Neuroscience

References & Citations

General Western Blot Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.