Anti-Gingipain B (RgpB) Antibody (13925)
Anti-Gingipain B (RgpB) Antibody (13925)
Product No.: 13925
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Clone 18E6.F7 Target Gingipain B Formats AvailableView All Product Type Monoclonal Alternate Names Arginine-gingipain B Isotype Mouse IgG1 Applications IHC , WB |
Data
RgpB coated on the solid phase at 4ug/ml. Antibody binding detected with HRP antimouse IgG Fc (1:2,000) and ABTS substrate.
Paraffin-embedded formalin-fixed AD hippocampus and gingival tissue from a patient with periodontal disease stained with #13925 and compared to non-AD control tissue and mouse IgG1 control (see Dominy et al. Science Advances 5 (1): eaau3333). 
Detection of RgpB in P. gingivalis wild-type strain W83 whole cells. A band of ~70-90kDa is detected. (see Nguyen K-A et al. J Bacteriol 189: 833-843). - -
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Antibody DetailsProduct DetailsReactive Species Poryphyromonas gingivalis Host Species Mouse Immunogen Arginine-gingipain B (RgpB) from Porphyromonas gingivalis. Product Concentration Lot Specific Formulation This monoclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.2 - 7.4 with no carrier protein or preservatives added. State of Matter Liquid Product Preparation Antibodies are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Upon initial thawing, appropriately aliquot and store at -80°C. For long-term storage, keep at -80°C. Avoid repeated freeze-thaw cycles. Country of Origin USA Shipping Next Day 2-8°C Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes a unique epitope within the immunoglobulin (Ig)-like domain of arginine-gingipain (RgpB). Background Arginine-gingipain B (RgpB) is a cysteine protease produced exclusively by Porphyromonas gingivalis, and it plays a central role in the pathogenicity of P. gingivalis in periodontal disease. Over the last several years, a possible association between Alzheimer disease (AD) and chronic periodontitis has been suggested based on detection of P. gingivalis infiltration in autopsy specimens from the brains of people with AD in association with neurons, tau tangles, and beta-amyloid as well as in cerebrospinal fluid of individuals diagnosed with AD.
Oral administration of a gingipain inhibitor to mice with established brain infection by P. gingivalis decreased the amount of P. gingivalis DNA in brain as well as the levels of beta-amyloid and tumor necrosis factor-α. Taken together, the data suggest that gingipain inhibitors might be an effective treatment of AD. Clinical trials of gingipain inhibitors are currently underway. NCBI Gene Bank ID UniProt.org References & Citations1) Dominy S et al. 2019. Porphyromonas gingivalis in Alzheimer’s disease brains: Evidence for disease causation and treatment with small-molecule inhibitors. Science Advances 5 (1): eaau3333.
2) Nguyen K-A et al. 2007. Does the importance of the C-terminal residues in the maturation of RgpB from Porphyromonas gingivalis reveal a novel mechanism for protein export in a subgroup of Gram-negative bacteria? J Bacteriol 189: 833-843. Technical Protocols  Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
