Anti-HCN4 Cyclic Nucleotide-Gated Channel [Clone S114-10]
Anti-HCN4 Cyclic Nucleotide-Gated Channel [Clone S114-10]
Product No.: 11509
Data
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (11509). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (11509) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN4 Antibody (D) Composite.
Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (11509). Tissue: hippocampus. Species: Human. Fixation: Bouin’s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (11509) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (11509). Tissue: frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (11509) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.
Western Blot analysis of Human T-HEK cell lysate showing detection of HCN4 protein using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (11509). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (11509) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. - -
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen Fusion protein aa 1019-1108 (cytoplasmic C-terminus) of rat HCN4 (accession no. Q9JKA7). Product Concentration Lot Specific Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze-thaw cycles.
Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-10ug/mL. A band of ~1300kDa is detected.Immunohistochemistry and
Immunocytochemistry: use at 0.1-1ug/mL Immunofluorescence: use at 1-10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: COS cell lysate transiently transfected with HCN4. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human, mouse, and rat HCN4. It does not cross-react with other HCNs. Background Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Hyperpolarization-activated cation channels of the HCN gene family, such as HCN4, play a crucial role in the regulation of cell excitability. They contribute to spontaneous rhythmic activity in the heart and brain. Antigen DetailsFunction Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) that regulate the rhythm of heart beat. May contribute to the native pacemaker currents in neurons (Ih) (By similarity). May mediate responses to sour stimuli. {PubMed:11675786}. NCBI Gene Bank ID UniProt.org Research Area Ion Channels References & CitationsTechnical ProtocolsFACS ICC IF   AM |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
