Anti-Human Alpha Defensin-5 (HD5) – Purified in vivo GOLD™ Functional Grade
Anti-Human Alpha Defensin-5 (HD5) – Purified in vivo GOLD™ Functional Grade
Product No.: D400
Clone 8C8 Target HD5 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names DEFA5; DEF5; HD-5 Isotype Mouse IgG2b κ Applications ELISA , IHC FFPE , in vivo , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen Recombinant Human HD5 (aa 20-94). Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829963 Applications and Recommended Usage? Quality Tested by Leinco WB ELISA Additional Applications Reported In Literature ? IHC FFPE Dot Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 8C8 recognizes human alpha-defensin 5 (HD5). Background The colon lies adjacent to the small intestine and has heavy bacterial colonization from ingested food and water that is often contaminated with bacteria. Remarkably, the small intestine has a low microbial density. A special type of epithelial cell, called a Paneth cell, can be found clustered at the base of the tubular glands that lie between the villi of the inner surface of the small intestine. These cells secrete defensins which have been shown to have activity against both Gram-positive and Gram-negative microbes.3 Six human α-defensins, a subfamily of defensin peptides characterized by their cysteine spacing and disulfide connectivity2, have been identified. Human Paneth cells express α-defensin 5 (HD5) along with HD6. Paneth cells are most numerous in the ileum and have many features similar to those of myeloid cells. They are multifaceted cells with a large quantity of apically-located eosinophilic secretory granules containing lysozyme and other antimicrobial factors which are released upon bacterial stimulation.2 In addition, these cells express tumor necrosis factor alpha (TNF-a), CD1, and CD15.1 Paneth cells do not store defensins as fully processed or active peptides, rather they store them as inactive propeptides.2 They are released as mature peptides after trypsin digestion. Trypsin is also secreted from the Paneth cell granules. It has been reported that disrupted α-defensin processing in murine Paneth cells has initiated a vulnerability to enteric infection.2 HD5 expression levels have been found to be negatively correlated to intestinal infection. Additionally, studies have shown HD5 to be a strong antagonist towards human Papillomavirus infection. Furthermore, low expression of HD5 is thought to play a role in Crohn’s disease.3 Anti-Human Alpha Defensin-5 (HD5) Clone 8C8 has been shown to recognize the propeptide and the partially processed forms of HD5. However, clone 8C8 has been reported to scarcely recognize the mature peptide. There was no reported cross reactivity with HD6, lysozyme, or sPLA2. Antigen Distribution HD5 is highly expressed in the secretory granules of Paneth cells of the ileum. PubMed NCBI Gene Bank ID UniProt.org Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone 8C8 in the context of in vivo mouse studies refers to a monoclonal antibody that specifically recognizes human defensin-5 (HD5), but its use is limited to recognizing the human peptideit does not recognize mouse defensin-5.
Key points for in vivo use:
Other clones: No evidence from the search results suggests that clone 8C8 refers to widely used anti-mouse cell marker antibodies (such as anti-CD8? or others), or other common in vivo reagents for mouse studies. If you intended another 8C8 clone for a different target, specifying the antigen would clarify. Summary: Based on the information provided, the sterile packaged Bcl-2 Antibody (8C8) should be stored at 4°C in the dark. Storage RequirementsThe product specification clearly indicates that upon receipt, the antibody should be stored immediately at the recommended temperature of 4°C and kept protected from light. This storage temperature falls within the typical refrigerated range of 2°-8°C that is commonly used for biological products to maintain their stability and prevent degradation. Shipping and HandlingThe antibody is shipped with polar packs to maintain cold chain integrity during transport, emphasizing the importance of maintaining proper temperature conditions from the point of manufacture through storage. Once received, immediate transfer to the appropriate storage temperature is critical for preserving the product's integrity and functionality. The 4°C storage requirement is standard for many antibody products, as this temperature effectively slows down degradation processes while preventing freezing damage that could occur at lower temperatures. The 8C8 antibody is most commonly cited in the literature as a monoclonal antibody against the L1 protein of vaccinia virus. In studies where 8C8 is used, other antibodies frequently employed include 39D4, M12B9, M2E9, and M7B6, all of which are also monoclonal antibodies targeting different epitopes on the L1 protein. Context and supporting details:
Table: Commonly used antibodies with 8C8 in L1 studies
Depending on the exact protein system, "8C8" can also refer to other monoclonal antibodies with different specificitiesfor example, anti-alpha defensin 5 in certain immunology contexts. However, in the context of vaccinia virus L1 studies, the above antibodies are the key comparators or controls used alongside 8C8. The key findings from scientific literature regarding clone 8C8 primarily relate to its use as a monoclonal antibody in immunological assays. The most relevant citations and findings are:
In summary, clone 8C8 is primarily cited as a monoclonal antibody used for immunophenotyping, immunohistochemistry, and flow cytometry, focusing on detection of proteins such as CD3? in pigs or Defensin alpha 5 in human tissues and cell lines. Direct citations typically reference its application as a diagnostic or research tool, not as a subject of biological discovery itself. References & Citations1. Porter, EM. et al. (1997) Infect Immun. 65(6):2389-95. 2. Ghosh, D. et al. (2002) Nat Immunol. 3(6):583-90. 3. Wehkamp, J. et al. (2019) Proc Natl Acad Sci U S A. 116(9): 3746–3751. 4. Schwaderer, AL. et al. (2012) PLoS One. 7(2):e31712. 5. Hains, D. et al. (2016) US Patent Application Publication No. US 2016/0069905 A1 6. Shen, B. et al. (2005) J Clin Pathol. 58(7):687-94. 7. Ranki, A. et al. (2015) Clin Immunol. 158(2):212-20. 8. Hulscher, JB. et al. (2016) Pediatr Res. 80(2):306-10. Technical Protocols IHC FFPE   Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
