Anti-Human Apoptosis Protease Activating Factor-1 (NT) (Apaf-1)

Anti-Human Apoptosis Protease Activating Factor-1 (NT) (Apaf-1)

Product No.: A157

[product_table name="All Top" skus="A157"]

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Target
Apoptosis Protease Activating Factor-1
Product Type
Polyclonal Antibody
Alternate Names
CED4
Applications
WB

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Product Size

Data

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Rabbit
Immunogen
PN:A174
Product Concentration
0.5 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human Apoptosis Protease Activating Factor-1 (Apaf-1) recognizes an epitope near the N-terminus of human, mouse and rat Apaf-1. This polyclonal antibody was purified using affinity chromatography.
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf-1 (for apoptosis protease-activating factor 1).1,2 Apaf-1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis.3 Apaf-1 can also associate with caspase-4 and caspase-8.4 Apaf-1 is ubiquitously expressed in human tissues.

Antigen Details

References & Citations

1. Zou, H. et al. (1997) Cell 90:405-13
2. Cecconi, F. et al. (1998) Cell 94:727-37
3. Li, P. et al. (1997) Cell 91:479-89
4. Hu, Y. et al. (1998) Proc Natl Acad Sci USA 95:4386-91
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.