Anti-Human Apoptosis Protease Activating Factor-1 (NT) (Apaf-1)

PN:A174

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human Apoptosis Protease Activating Factor-1 (Apaf-1) recognizes an epitope near the N-terminus of human, mouse and rat Apaf-1. This polyclonal antibody was purified using affinity chromatography.

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf-1 (for apoptosis protease-activating factor 1).^1,2 Apaf-1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis.³ Apaf-1 can also associate with caspase-4 and caspase-8.⁴ Apaf-1 is ubiquitously expressed in human tissues.

1. Zou, H. et al. (1997) Cell 90:405-13
2. Cecconi, F. et al. (1998) Cell 94:727-37
3. Li, P. et al. (1997) Cell 91:479-89
4. Hu, Y. et al. (1998) Proc Natl Acad Sci USA 95:4386-91