Anti-Human Beta-Site APP Cleaving Enzyme 2 (CT) (BACE2)

PN:B366

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human Beta-Site APP Cleaving Enzyme 2 (BACE2) recognizes an epitope near the C-terminus of human, mouse and rat BACE2. This polyclonal antibody was purified using affinity chromatography.

Accumulation of the amyloid-β (αβ) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. αβ peptide is generated by proteolytic cleavage of the β-amyloid protein precursor (APP) at β- and γ-sites by proteases. The long-sought β-secretase was recently identified by several groups independently and designated β-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2).^1-4 A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 1.^4-9 BACE2 also cleaves APP at β-site and at a different site within αβ.⁸ BACE2 locates on chromosome 21q22.3, the so-called ‘Down critical region’, suggesting that BACE2 and αβ may also contribute to the pathogenesis of Down syndrome.⁶,⁷

1. Vassar R, et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999; 286:735-41. 2. Hussain I, et al. Identification of a novel aspartic protease (Asp2) as beta-secretase. Mol. Cell. Neurosci. 1999; 14:419-27. 3. Sinha S, et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999; 402:537-40. 4. Yan R, et al. Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity. Nature 1999; 402:533-7. 5. Lin X, et al. Human aspartic protease memapsin 2 cleaves the beta-secretase site of beta-amyloid precursor protein. Proc. Natl. Acad. Sci. USA 2000 15; 97:1456-60. 6. Acquati F, et al. The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the down critical region. FEBS Lett. 2000; 468:59-64. 7. Solans A, et al. A new aspartyl protease on 21q22.3, BACE2, is highly similar to Alzheimer's amyloid precursor protein betasecretase. Cytogenet Cell Genet. 2000; 89:177-84. 8. Farzan M, et al. BACE2, a beta -secretase homolog, cleaves at the beta site and within the amyloid-beta region of the amyloid-beta precursor protein. Proc. Natl. Acad. Sci. USA 2000; 97:9712-7. 9. Bennett BD, et al. Expression analysis of BACE2 in brain and peripheral tissues. J. Biol. Chem. 2000; 275:20647-51. (06-01D)