Pricing & Details

Product No.B458
Protein
Bik
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Bcl-2-interacting killer, apoptosis inducer NBK
Applications
IHC FFPE
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
B458-20 µg
20 µg
$95.00
In stock
Max:
Min: 1
Step: 1
B458-0.1 mg
0.1 mg
$440.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
PN:B489
Product Concentration
0.5 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human Bik recognizes human and mouse Bik. This polyclonal antibody was purified using affinity chromatography.
Background
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such protein BIK was recently identified as an endoplasmic reticulum (ER)-residing pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family that stimulates mitochondrial release of cytochrome c following p53 induction of apoptosis. A significant fraction of BIK is found as an ER transmembrane protein, with most of the protein facing the cytosol. Restricting BIK to the ER membrane by replacing the transmembrane region with that of the ER-selective membrane anchor of cytochrome b5 resulted in a decreased cytochrome c release from mitochondria and a corresponding drop in cell death. Recent evidence suggests that BIK cooperates with NOXA, another BH3-only protein, to somehow enhance the activation of Bax to stimulate the rapid release of cytochrome c from mitochondria.

Antigen Details

Protein
PubMed

References & Citations

1. Lockshin, RA. et al. (2000) Cell Death Differ. 7:2-7.
2. Germain, M. et al. (2002) J. Biol. Chem. 277(20):18053-60.
3. Germain, M. et al. (2005) EMBO J. 24:1546-56.
IHC
General Western Blot Protocol
Prod No.
Description
B458
B489
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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